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Determinants of Bone and Muscle Quality and Strength in Obesity With and Without Diabetes

Completed
Conditions
Obesity
Fractures, Bone
Osteoporotic Fractures
Osteoporosis
Musculoskeletal Diseases
Diabetes Mellitus
Diabetes Mellitus, Type 2
Fall
Body Weight
Bone Diseases, Metabolic
Registration Number
NCT04093856
Lead Sponsor
Suzanne Morin
Brief Summary

Background: Osteoporotic fractures are a major public health issue. They cause substantial disability, loss of autonomy, morbidity and excess mortality. Diabetes is also associated with increased risk for falls and fractures through a direct impact of elevated blood glucose on the skeleton and on muscles.

Research project overview: The investigators propose a cross-sectional study that will involve 2 research centers in the province of Quebec. The investigators will recruit 20 obese participants, without diabetes, who have not undergone bariatric surgery, for one-time measurements to be compared with baseline measurements (pre-surgery) from participants in the bariatric obese diabetic groups with type II diabetes mellitus from the ongoing study BODI study (NCT03455868). Bone Mineral Density as well as muscle quality, strength and function will be evaluated at a single study visit.

Relevance: This data will permit the evaluation of the bone-muscle unit in patients with obesity with and without diabetes, and assess whether the presence and duration of diabetes impacts further on clinical and functional musculoskeletal outcomes (falls, fractures and mobility and strength) in this population. AGEs, if associated with muscle and bone deterioration, might become an easily accessible biomarker of musculoskeletal health in the clinical setting.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
40
Inclusion Criteria

A) Diabetic group

  • BMI >=35 kg/m2
  • Clinical diagnosis of type 2 diabetes
  • Use of oral hypoglycemic agents or insulin or 2 of the following tests confirming type 2 diabetes: HbA1c >=6.5%; fasting glucose >=7.0 mM; 2-h glucose post 75g oral glucose tolerance test (OGTT) >=11.1 mM)

B) Non-diabetic group:

  • BMI >=35 kg/m2
  • Normoglycemia (HgbA1c <5.7% and Fasting glucose <5.6 nM)
Exclusion Criteria
  • BMI >60 kg/m2
  • Clinical diagnosis of type 1 diabetes
  • Disease (e.g. uncontrolled thyroid disease, malabsorptive or overt inflammatory disorder, metabolic bone disease, creatinine clearance <60 ml/min)
  • Medication (e.g. gluccocorticoids, anti-epileptic drugs, osteoporosis therapy and thiazolidinediones) affecting bone metabolism
  • Pregnancy
  • History of oesophageal, gastric, digestive or bariatric surgery
  • Prosthesis that could interfere with interpretation of imaging data
  • Chronic severe condition or illness precluding from participation in the project.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Muscle composition measured as muscle fat content at the proximal femurBaseline

Measured by quantitative computed tomography (QCT)

Muscle size measured as cross-sectional muscle area at the proximal femurBaseline

Measured by quantitative computed tomography (QCT)

Volumetric bone mineral density (vBMD) at the spine, hip, radius and tibiaBaseline

Measured by quantitative computed tomography (QCT)

Secondary Outcome Measures
NameTimeMethod
Lower extremity muscle strength measured by peak torque generated by knee extensionBaseline

Measured using Biodex isokinetic dynamometer

Functional mobility measured by time that a person takes to rise from a chair, walk three meters, turn around, walk back to the chair, and sit down.Baseline

Measured using Timed Up and Go test.

Functional exercise capacity measured by total distance ambulated during a 6 minute time periodBaseline

Measured using Six Minute Walk Test

Leptin concentrationBaseline

Hormone involved in bone metabolism

Physical healthBaseline

SF-12

Osteocalcin (total and decarboxylated) concentrationBaseline

Serum bone formation marker

Bone specific alkaline phosphatase concentrationBaseline

Serum bone formation marker

N-terminal propeptide of type 1 procollagen concentrationBaseline

Serum bone formation marker

C-telopeptide concentrationBaseline

Serum bone resorption marker

Insulin concentrationBaseline

Hormone involved in bone metabolism

Parathormone concentrationBaseline

Hormone involved in bone metabolism

Estradiol concentrationBaseline

Hormone involved in bone metabolism

Body composition measured as visceral and subcutaneous adipose tissueBaseline

Measured by computed tomography (CT)

Upper extremity muscle strength measured by grip force in kgBaseline

Measured using Jamar Hydraulic Hand Dynamometer

Sclerostin concentrationBaseline

Hormone involved in bone metabolism

Fasting Hemoglobin A1c concentrationBaseline

Diabetes control

Body composition measured by fat and lean massBaseline

Measured using dual-energy X-ray absorptiometry (DXA)

Bone marrow adiposityBaseline

Measured by computed tomography (CT)

Cutaneous Advanced glycation end products (AGEs) measurementBaseline

Measured by measured by AGE Reader

Fasting glucose concentrationBaseline

Diabetes control

Serum 25-hydroxyvitamin D concentrationBaseline

Hormone involved in bone metabolism

Insulin like growth factor-1 concentrationBaseline

Hormone involved in bone metabolism

Adiponectin concentrationBaseline

Hormone involved in bone metabolism

Self-reported physical activity measured in metabolic equivalents (MET)Baseline

Measured using the International Physical Activity Questionnaire

Functional balance score obtained using multidimensional balance assessment. Higher scores indicate better balance abilitiesBaseline

Fullerton Advanced Balance (FAB) Scale

Physical activity measured by total activity and time spent in different intensities of exercise measured over 7 consecutive daysBaseline

Actigraph GT3X+ Accelerometer

Community Participation RestrictionBaseline

Life-space mobility assessment

Health-related quality of lifeBaseline

How are you today? Visual analogue scale

Trial Locations

Locations (2)

Research Institute of the McGill University Health Centre

🇨🇦

Montréal, Quebec, Canada

Centre de recherche de l'IUCPQ

🇨🇦

Québec, Canada

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