Phase I Study of Adoptive Immunotherapy for Advanced ROR1+ Malignancies With Defined Subsets of Autologous T Cells Engineered to Express a ROR1-Specific Chimeric Antigen Receptor
Overview
- Phase
- Phase 1
- Intervention
- Not specified
- Conditions
- Hematopoietic and Lymphoid Cell Neoplasm
- Sponsor
- Fred Hutchinson Cancer Center
- Enrollment
- 21
- Locations
- 1
- Primary Endpoint
- Number of Participants That Experienced Adverse Events
- Status
- Terminated
- Last Updated
- 3 years ago
Overview
Brief Summary
This phase I trial studies the side effects and best dose of genetically modified T-cell therapy in treating patients with receptor tyrosine kinase-like orphan receptor 1 positive (ROR1+) chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), stage IV non-small cell lung cancer (NSCLC), or triple negative breast cancer (TNBC) that has spread to other places in the body and usually cannot be cured or controlled with treatment (advanced). Genetically modified therapies, such as ROR1 specific chimeric antigen receptor (CAR) T-cells, are taken from a patient's blood, modified in the laboratory so they specifically may kill cancer cells with a protein called ROR1 on their surfaces, and safely given back to the patient after conventional therapy. The "genetically modified" T-cells have genes added in the laboratory to make them recognize ROR1.
Detailed Description
PRIMARY OBJECTIVE: I. To evaluate the safety of adoptive T cell therapy using ex vivo expanded autologous cluster of differentiation (CD)8+ and CD4+ ROR1 CAR-T cells for patients with advanced ROR1+ hematologic (Cohort A) and epithelial (Cohort B) malignancies. SECONDARY OBJECTIVES: I. To determine duration of in vivo persistence of adoptively transferred T cells, and the phenotype of persisting T cells. II. To determine trafficking of adoptively transferred T cells traffic to the bone marrow or other tumor site and function in vivo. III. To determine preliminary antitumor activity of the adoptive transfer of ROR1 CAR-T cells in patients with measurable tumor burden prior to T cell transfer. OUTLINE: This is a dose escalation study of ROR1 CAR-specific autologous T-lymphocytes. Patients receive chemotherapy comprising fludarabine phosphate and cyclophosphamide as determined by the referring physician in consultation with the protocol principal investigator (PI). Beginning within 36-96 hours after completion of lymphodepleting chemotherapy, patients receive ROR1 CAR-specific autologous T-lymphocytes intravenously (IV) over 20-30 minutes. Patients may receive a second infusion of ROR1 CAR-specific autologous T-lymphocytes with or without additional cytoreductive therapy at the same (for those that received the highest cell dose) or up to the next highest dose level and there is persistent disease, there were no toxicities attributed to the first infusion, and the patient is at least 21 days from the first T cell infusion. After completion of study treatment, patients are followed up for at least 15 years.
Investigators
David Maloney
Professor, Clinical Research Division
Fred Hutchinson Cancer Center
Eligibility Criteria
Inclusion Criteria
- •INCLUSION CRITERIA FOR PATIENTS WITH CLL, MCL OR ALL (COHORT A)
- •CLL who are beyond first remission and who have failed combination chemoimmunotherapy with regimens containing a purine analogue and anti-CD20 antibody, or who have failed tyrosine kinase or phosphatidylinositol 3 (PI3) kinase inhibitors, or who were not eligible for or declined such therapy; patients with fludarabine refractory disease are eligible
- •Mantle cell lymphoma patients who are beyond first remission and previously treated with chemoimmunotherapy; patients who have relapsed following autologous hematopoietic cell transplant (HCT) are eligible
- •ALL patients who have relapsed or have residual disease following treatment with curative intent; ALL patients must have ROR1 expressed on \> 90% of the leukemia blasts to be eligible
- •Confirmation of diagnosis by internal pathology review of initial or subsequent biopsy or other pathologic material at the Fred Hutchinson Cancer Research Center (FHCRC)/Seattle Cancer Care Alliance (SCCA)
- •Evidence of ROR1 expression by immunohistochemistry or flow cytometry on any prior or current tumor specimen
- •Karnofsky performance status \>= 70%
- •Negative pregnancy test for women of childbearing potential; subjects of childbearing potential are those who have not been surgically sterilized or have not been free from menses for \> 1 year
- •Fertile male and female patients must be willing to use a contraceptive method before, during, and for at least two months after the T cell infusion
- •Ability to understand and provide informed consent
Exclusion Criteria
- •EXCLUSION CRITERIA FOR PATIENTS WITH CLL, MCL, OR ALL (COHORT A)
- •Treatment with other investigational agent(s) within 30 days of planned lymphodepletion
- •Patients requiring ongoing daily corticosteroid therapy at a dose of \> 15 mg of prednisone per day (or equivalent); pulsed corticosteroid use for disease control is acceptable
- •Active autoimmune disease requiring immunosuppressive therapy
- •Serum creatinine \> 2.5 mg/dL
- •Serum glutamic oxaloacetic transaminase (SGOT) \> 5 x upper limit of normal
- •Bilirubin \> 3.0 mg/dL
- •Patients with clinically significant pulmonary dysfunction, as determined by medical history and physical exam should undergo pulmonary function testing; those with a forced expiratory volume in 1 second (FEV1) of =\< 65% or diffusion capacity of the lung for carbon monoxide (DLCO) (corrected) \< 40% will be excluded
- •Significant cardiovascular abnormalities as defined by any one of the following: congestive heart failure, clinically significant hypotension, symptomatic coronary artery disease, or a documented ejection fraction of \< 45%; any patient with an ejection fraction (EF) of 45-49% must receive clearance by a cardiologist to be eligible for the trial
- •Patients who are human immunodeficiency virus (HIV) seropositive
Outcomes
Primary Outcomes
Number of Participants That Experienced Adverse Events
Time Frame: Within 35 days of receptor tyrosine kinase-like orphan receptor 1 positive chimeric antigen receptor-T cell infusion
Will be graded according to Common Terminology Criteria for Adverse Events. Outcome will be reported as a count of participants that experienced adverse events in each arm.
Secondary Outcomes
- Number of Participants Biopsied With Detectable CD3 T-cells(Up to 48 days post infusion)
- Number of Participants With Persistence of Adoptively Transferred Receptor Tyrosine Kinase-like Orphan Receptor 1 Positive Chimeric Antigen Receptor-T Cells(Up to 28 days after the T cell infusion)
- Objective Response Rate of Complete Remission and Partial Remission(Up to 1 year)
- Progression Free Survival(Up to 1 year)
- Overall Survival(Up to 1 year)