Effects of DHA on Post-operative Fibrosis of Trabulectomy Blebs in Glaucoma Surgery
- Conditions
- Deep SclerectomyTrabulectomy
- Interventions
- Procedure: Samples of fibroblasts from Tenon's capsule
- Registration Number
- NCT03289806
- Lead Sponsor
- Centre Hospitalier Universitaire Dijon
- Brief Summary
Post-operative fibrosis of trabulectomy blebs is a major cause of surgical failure leading to a renewed increase in intraocular pressure. The use of anti-metabolites over many years has made it possible to improve success rates for this surgery but these drugs have numerous adverse effects as they are toxic. Alternative therapies are thus necessary, and we believe that DHA could have antifibrotic effects on fibroblasts and could thus improve success rates in this surgery.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 20
Inclusion Criteria
- Patients undergoing trabulectomy or deep sclerectomy
- Patients informed about the study
Exclusion Criteria
- Patients who refuse to take part in the study
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Controls Samples of fibroblasts from Tenon's capsule Strabismus surgery Cases Samples of fibroblasts from Tenon's capsule Glaucoma surgery
- Primary Outcome Measures
Name Time Method Test of cell viability by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) At baseline Test proliferation with immunohistochemistry (Ki67 staining) At baseline Test migration ability by video microscopy At baseline Analysis of cell cycle in flow cytometry At baseline
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Chu Dijon Bourogne
🇫🇷Dijon, France