The Effect of Supplementation of Vitamin D3 on Inflammation Induced by 100 km Running, Iron Metabolism and Erythropoiesis
- Conditions
- Inflammation; MuscleHealthyIron MetabolismIneffective Erythropoiesis
- Interventions
- Other: placebo
- Registration Number
- NCT05880030
- Lead Sponsor
- Gdansk University of Physical Education and Sport
- Brief Summary
A group of runners received vitamin D (10,000 IU - international unit per day) for two weeks. The aim of the intervention was to check the effect of vitamin D supplementation on selected parameters of inflammation and iron metabolism in comparison with the placebo group. Blood was collected before and after supplementation. Next: before, after 25, 50, 75,100 km running and 12 hours after the run. The data were subjected to statistical analysis.
- Detailed Description
Subjects were randomized to two groups: the experimental one, n = 20 and the placebo one, n = 20 with the same age, experience in running and 25(OH)D level. The experimental group received vitamin D3 supplementation (around 10,000 UI/day, i.e., 20 droplets -Vigantol medicament, Merck) and the control group received a placebo (20 droplets of sunflower oil, placed in an identical bottle as vitamin D3) for two weeks before a run. The last vitamin D dose was taken 24 h before the 100 km ultra-marathon. 40 male athletes took part in the 100 km ultra-marathon. Blood was collected before (day 0) and after supplementation (day 14). Next (day 15): before, after 25, 50, 75,100 km running and 12 hours after the run (day 16). The data were subjected to statistical analysis.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 40
- male
- athletes running ultra-marathons
- vitamin D3 supplementation
- multivitamin and iron supplementation
- serum 25(OH)D low < 10 ng/mL
- serum 25(OH)D high > 100 ng/mL
- haemoglobin < 10 g/dL
- other (sports injury, tournament)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Control placebo sunflower oil, (20 droplets) two weeks supplementation. Vitamin D Cholecalciferol Vigantol 10 000 IU/day (20 droplets) two weeks supplementation (vitamin D3)
- Primary Outcome Measures
Name Time Method Observation of changes in creatine kinase level induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured parameter: creatine kinase \[U/l\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
The change in parathyroid hormone level after two-week supplementation. Day 0 to day 14 Measured parameter: parathyroid hormone \[pg/ml\].
The change in vitamin 25(OH)D level after two-week supplementation. Day 0 to day 14 Measured parameter: vitamin 25(OH)D \[ng/ml\].
Observation of changes in inflammatory parameters: C reactive protein - CRP induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured parameter of inflammatory: CRP\[mg/l\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
Observation of changes in leucocytes induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured parameters of inflammatory: leukocytes \[G/l\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
Observation of changes in inflammatory parameters:interleukin-6 induced by a 100 km run Day "15 - the run" and day "16 - 12 hours after the run" Measured parameter of inflammatory: interleukin-6 \[pg/ml\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
- Secondary Outcome Measures
Name Time Method Observation of changes in reticulocytes induced by a 100 km run. Day "15 - the run" and day "16 - 12 hours after the run" Measured reticulocytes \[promil\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12 hours after the run (06 11 2016).
Observation of changes in erythroferrone level induced by a 100 km run. Day "15 - the run" and day "16 - 12 hours after the run" Measured erythroferrone level \[pg/ml\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12 hours after the run (06 11 2016).
Observation of changes in hepcidin level induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured parameters of iron metabolism: hepcidin \[ng/mL\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
Observation of changes in serum iron level induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured serum iron level \[µg/dL\].Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
Observation of changes in ferritin induced by 100 km running. Day "15 - the run" and day "16 - 12 hours after the run" Measured parameters of iron metabolism: ferritin \[ng/mL\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12h after the run (06 11 2016).
Observation of changes in hematocrit induced by a 100 km run. Day "15 - the run" and day "16 - 12 hours after the run" Measured hematocrit \[%\].Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12 hours after the run (06 11 2016).
Observation of changes in erythrocytes level induced by a 100 km run. Day "15 - the run" and day "16 - 12 hours after the run" Measured erythrocytes level \[T/l\].Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12 hours after the run (06 11 2016).
Observation of changes in erythropoietin level induced by a 100 km run. Day "15 - the run" and day "16 - 12 hours after the run" Measured erythropoietin level \[mIU - milli-International unit/mL\]. Collected blood samples before and after 25, 50, 75 and 100-km run (05 11 2016) and 12 hours after the run (06 11 2016).