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Effect of Lactobacillus Paracasei PS23 on Improving Aging and Muscle Loss in the Elderly

Not Applicable
Completed
Conditions
Depression
Muscle Loss
Anxiety
Elderly
Registration Number
NCT06062472
Lead Sponsor
Chi-Chang Huang
Brief Summary

This study refers to the supplementation of PS23 in human experiments to help the elderly maintain muscle mass, delay muscle loss, promote physical activity, and delay the occurrence of disability and debilitation in elderly people.

Detailed Description

Taiwan has become an aging society in recent years. There are important issues to attenuate aging and activate the potential capacity of elderly population. The physical function of middle-aged and older people will degenerate with age and produce age-related diseases. Common symptoms are organ function deterioration, and old age disability caused by malnutrition and muscle loss is also a common care problem. Therefore, how to prevent the complications of muscle loss is one of the important ten years of geriatric research. Although past research has been confirmed, it can improve the debilitating condition of the elderly through nutrition counseling and exercise intervention. However, there is not much in-depth discussion on how probiotics can improve muscle loss and delay symptoms in elderly people. Therefore, this study refers to the supplementation of PS23 in human experiments to help the elderly maintain muscle mass, delay muscle loss, promote physical activity, and delay the occurrence of disability and debilitation in elderly people. One hundred and twenty elderly people will be recruited and then divided into three groups with 40 people in each group: (A) placebo group, (B) PS23 probiotic group, (C) PS23-HT prebiotic group, supplemented for 12 weeks. The diet record, functional test and muscle mass will be carried out every six weeks after the start, to understand whether PS23 is effective in improving muscle loss and weakness in elderly people.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
119
Inclusion Criteria
  • age between 65 and 85 years old
Exclusion Criteria
  • Have taken antibiotics within one month or are receiving antibiotic treatment
  • Have used probiotic products in powder, capsule or tablet form within two weeks (except for yogurt, yogurt, Yakut and other related foods)
  • People allergic to lactic acid bacteria products
  • Patients diagnosed with stroke or hypertension (systolic blood pressure greater than 139, diastolic blood pressure greater than 89)
  • Exclusion of limbs and hips that have been replaced with artificial joints
  • Persons with incapacity/insufficient decision-making ability
  • Who has been determines that it is not suitable to participate in the research.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Primary Outcome Measures
NameTimeMethod
30 seconds chair stand testFrom Baseline, 6 weeks and 12 Weeks Assessed

30 seconds chair stand test will be used as a self-test. Number of stands will be counted.

muscle massFrom Baseline, 6 weeks and 12 Weeks Assessed

Use dual-energy X-ray absorptiometry, DXA (Lunar iDXA, GE Healthcare, IL, USA) for no-entry measurement to determine muscle mass

Grip strengthFrom Baseline, 6 weeks and 12 Weeks Assessed

Use the armed grip machine to measure, hold each of the left and right hands three times until they are weak, and record the maximum value

Secondary Outcome Measures
NameTimeMethod
Stress assessment - difference of Testosterone levelFrom Baseline, 6 weeks and 12 Weeks Assessed

To assess inflammation indicators, used commercial kit human Testosterone (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis Male 265-923 (in ng/dl) Female 15-70 (in ng/dl) for 19 years and older

Gut microbiotaFrom Baseline 12 Weeks Assessed

Collect the DNA of fecal flora to test the diversity and abundance of gut microbiota in Stool samples before and after probiotics

Visual Analogue Scale for GI symptoms, VAS-GIFrom Baseline, 6 weeks and 12 Weeks Assessed

Visual Analogue Scale for GI symptoms, VAS-GI (visual analogue scale, VAS 0-10) was designed to measure the response of symptoms and well-being in patients after taking probiotics

Get Up and Go testFrom Baseline, 6 weeks and 12 Weeks Assessed

To evaluate functional walking ability

Sit and Reach TestFrom Baseline, 6 weeks and 12 Weeks Assessed

To evaluate lower-limb strength and flexibility

Safety assessment - BunFrom Baseline, 6 weeks and 12 Weeks Assessed

Safety is assessed function of liver and kidney such as Bun (6-20 mg/dl)

Q-LES-QFrom Baseline, 6 weeks and 12 Weeks Assessed

Participants who receive app-CBT will have greater improvement on quality of life, assessed using The Quality of Life, Enjoyment, and Satisfaction Questionnaire - Short Form (Q-LES-Q). The Q-LES-Q-SF is a self-report measure of subjective quality of life, containing Likert items ranging from 1 (Very Poor) to 5 (Very Good). Total scores are presented as a percentage of the maximum value (i.e., ranging from 0 to 100, with higher scores indicating greater quality of life).

Safety assessment - ASTFrom Baseline, 6 weeks and 12 Weeks Assessed

Safety is assessed function of liver and kidney such as AST (8-38 IU/L).

Safety assessment - ALTFrom Baseline, 6 weeks and 12 Weeks Assessed

Safety is assessed function of liver and kidney such as ALT (4-44 IU/L)

Change in Insulin SensitivityFrom Baseline, 6 weeks and 12 Weeks Assessed

Change in fasting glucose

fat percentageFrom Baseline, 6 weeks and 12 Weeks Assessed

Use dual-energy X-ray absorptiometry, DXA (Lunar iDXA, GE Healthcare, IL, USA) for no-entry measurement to determine fat percentage

One-leg stance testFrom Baseline, 6 weeks and 12 Weeks Assessed

To evaluate static balance ability

Safety assessment - CreatinineFrom Baseline, 6 weeks and 12 Weeks Assessed

Safety is assessed function of liver and kidney such as Creatinine (0.6-1.3 mg/dl)

Difference in Oxidative stress - blood level of HSCRPFrom Baseline, 6 weeks and 12 Weeks Assessed

To assess inflammation indicators, used commercial kit human CRP (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis

The serum IL-6 levelFrom Baseline, 6 weeks and 12 Weeks Assessed

the measurement of serum IL-6 level in blood

Generalized Anxiety Disorder 7-Item, GAD-7From Baseline, 6 weeks and 12 Weeks Assessed

The GAD-7 Anxiety Questionnaire involves 7 items assessing how often participants have felt bothered by anxiety-related problems over the past two weeks. Each item is rated on a Likert scale from 0 (not at all) to 3 (nearly every day). The total score is the sum of responses and ranges from 0-21; where 0-4 = minimal anxiety; 5-9 = mild anxiety; 10-14 = moderate anxiety; 15-21 = severe anxiety

Geriatric Depression Scale, GDSFrom Baseline, 6 weeks and 12 Weeks Assessed

It consists of 30 questions questioning the mood in the last week. This short form consists of 15 questions questioning the mood of the patient. The answers are given according to the feelings in the last week; The answers are "yes" or "no" and 1 point is given for "yes" or "no" answers depending on the question. For the long form: Normal 0 - 10, Mild 11 - 20, Moderate to Severe 21 - 30.For the short form: Normal 0 - 4, Mild 5 - 9, Moderate to Severe 10 - 15.

PGI-CFrom Baseline to 12 Weeks Assessed

The PGIC is a scale participants use to rate the level of change they have experienced following treatment. The PGIC is a 7-point scale from 1 (very much better) to 7 (very much worse).

10 meter walkFrom Baseline, 6 weeks and 12 Weeks Assessed

Use a pyramid and a measuring tape to measure a distance of ten meters, and ask the subject to stand up from the chair, go around the pyramid and sit down, and record the time

Cardiorespiratory fitnessFrom Baseline, 6 weeks and 12 Weeks Assessed

2-min step test to assess the functional capacity of the respiratory and cardiovascular systems

Level of Growth Differentiation Factor-15From Baseline, 6 weeks and 12 Weeks Assessed

Level of Growth Differentiation Factor-15

TNF-a determination using ELISAFrom Baseline, 6 weeks and 12 Weeks Assessed

A serum level of TNF-a was determined by quantitative sandwich enzyme immunoassay (R\&D Systems, Inc., Minneapolis, MN, USA) guided by to the manufacturer's instructions. The intensity of the color was measured at 490 nm.

Trial Locations

Locations (1)

National Taiwan Sport University

🇨🇳

Taoyuan, Taiwan

National Taiwan Sport University
🇨🇳Taoyuan, Taiwan

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