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Presepsin in the Diagnosis of Sepsis in Critically Ill Patients

Completed
Conditions
Infection
Sepsis
Interventions
Diagnostic Test: Presepsin measurement
Registration Number
NCT03584594
Lead Sponsor
University Hospital Ostrava
Brief Summary

Sepsis is one of the most common causes of death worldwide. It is caused by a complex of inadequate host responses to infection. Sepsis remains a major challenge of modern intensive care medicine. Despite recent improvements, the incidence of sepsis in critically ill patients increases steadily (25%) and mortality rates remain unacceptably high (30%). It is difficult to distinguish the sepsis from the non-infectious systemic inflammatory response syndrome. Early identification of the origin of infection can help dramatically to improve outcome and reduce mortality. That is why clinicians need fast, reliable and specific biomarkers for sepsis recognition.

Detailed Description

Comparison between the detection of novel early inflammatory biomarker (PSEP) and the others normally used biomarkers (c-reactive protein - CRP, interleukin 6 - IL6, procalcitonin - PCT) in the early diagnosing of sepsis in the critically ill patients A broad range of clinical and laboratory parameters are combined (Surviving sepsis campaign, international guidelines) for early sepsis identification: white blood cells (WBC), C-reactive protein (CRP), interleukin 6 (IL-6), procalcitonin (PCT).

An ideal biomarker should be a fast and specific increase in sepsis, short half-life, rapid decrease after administration of an effective therapy and fast (bed-side) method of determination. None of the current biomarkers have all of these characteristics.

We investigate the diagnostic accuracy of presepsin compared to other biomarkers (WBC, PCT, IL6, CRP) for infection or sepsis, defined according to Sepsis-3 definition (Singer, JAMA 2016) in adult patients admitted to ICU with suspected sepsis.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
200
Inclusion Criteria
  • signed informed consent
  • diagnosis of sepsis from qSOFA (quick Subsequent Organ Failures Assessment)
  • need of vasopressors for mean arterial pressure (MAP) ≥ 65 mmHg
  • lactate levels ≥ 2mmol/l despite adequate volume resuscitation
Exclusion Criteria
  • age below 18 years
  • terminal state of disease
  • pregnancy

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Presepsin assessmentPresepsin measurementResidual blood samples after performing all necessary blood examinations and analyses will be used to determine the level of presepsin, as the potential new biomarker of infection.
Primary Outcome Measures
NameTimeMethod
Serum concentration of Presepsin47 months

Serum concentration of Presepsin in patients with sepsis or septic shock will be compared to PCT, IL6 and CRP results.

Area under the Receiver-operating characteristic Curve47 months

Area under the Receiver-operating characteristic Curve (ROC-AUC) of the presepsin and other biomarkers (PCT, IL6, CRP) for diagnostic value of any biomarker will be analysed on a scale 0-100.

Secondary Outcome Measures
NameTimeMethod
Correlation of serum concentration of presepsin with detection of microbial agents47 months

Comparison of presepsin concentration in serum with the results of culture/microscopy of a pathogen from a clinical focus using methods of classical microbiology. In patients with risk factors for invasive mycosis, comparison of presepsin concentration with serum for galactomannan (GM) and beta- D- glucan (BG) detection will be performed.

Trial Locations

Locations (2)

Public Health Institute Ostrava

🇨🇿

Ostrava, Moravian-Silesian Region, Czechia

University Hospital Ostrava

🇨🇿

Ostrava, Moravian-Silesian Region, Czechia

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