Stem Cell Recruitment in Exercised Skeletal Muscles of Older Women by Phytosteroid Rg1

Not Applicable

Completed

• Conditions: Exercise Training; Post-menopausal Women; Bone Marrow Stem Cells; Skeletal Muscle Recovery

• Registration Number: NCT07093190
• Lead Sponsor: University of Taipei

Brief Summary

The goal of this interventional study is to observe the effects of bone marrow stem cell behavior in skeletal muscle of women between 60 to 75 years old after a single moderate resistance exercise session. Plant sterol Rg1, a compound known to enhance stem cell infiltration following exercise in young men will be tested against a placebo in a double blinded crossover design.

The main questions to be answered are:

* Does Rg1 supplementation alter serum sex hormone levels in recovery from exercise?

* Does Rg1 supplementation influence bone marrow stem cell differentiation in skeletal muscles of older women?

Participants will:

* Pre-test body composition, maximal leg strength, blood and muscle tissue.

* One hour after taking the supplement, participants will perform 4 sets of 10 repetitions of 70% of maximal strength (1RM) leg press and have two thigh muscle biopsies (0 and 24 h after exercise).

* Repeat the protocol one month later on the alternative supplement. Researchers will understand whether Rg1 improves recovery response to resistance exercise in post-menopausal women.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-03-18
• Primary Completion: 2022-12-02
• Status Verified: 2025-07
• Study Completion: 2025-07-01
• Study First Submitted: 2025-07-21
• Study First Submitted QC: 2025-07-21
• Last Update Submitted: 2025-07-21
• Study First Posted: 2025-07-30
• Last Update Posted: 2025-07-30

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 11

Inclusion Criteria

• sedentary, able to cease medications and supplements for 48 h before and 24 h after exercise session, medically sound for lower body moderate resistance exercise

Exclusion Criteria

• use of insulin, sex hormone and immune-modulating medications, smoking and regular alcohol consumption

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Nestin+ neural stem cell area in skeletal muscle biopsies	from enrolment to the end of study at 8 weeks	Immunofluorescence staining will be used to semi-quantitatively analyze Nestin+ neural stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
Stro-1+ bone marrow stem cell area in skeletal muscle biopsies	from enrolment to the end of study at 8 weeks	Immunofluorescence staining will be used to semi-quantitatively analyze Stro-1+ BMSC presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
CD34+/CD31+ vascular stem cell area in skeletal muscle biopsies	from enrolment to the end of study at 8 weeks	Immunofluorescence staining will be used to semi-quantitatively analyzeCD34+/CD31+ vascular stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
Serum sex hormone levels 24 h following resistance exercise	from enrolment to the end of study at 8 weeks	Forearm blood draw and Elisa analysis of serum for estradiol, progesterone, and testosterone of older women before and 24h after a moderate resistance exercise session.

Trial Locations

Locations (1)

University of Taipei; 🇨🇳 Taipei, Taiwan