Effect of Rg1 Supplementation on Stem Cells in Exercised Skeletal Muscles of Women > 60 y
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 11
- 试验地点
- 1
- 主要终点
- Nestin+ neural stem cell area in skeletal muscle biopsies
研究概览
简要总结
The goal of this interventional study is to observe the effects of bone marrow stem cell behavior in skeletal muscle of women between 60 to 75 years old after a single moderate resistance exercise session. Plant sterol Rg1, a compound known to enhance stem cell infiltration following exercise in young men will be tested against a placebo in a double blinded crossover design.
The main questions to be answered are:
- Does Rg1 supplementation alter serum sex hormone levels in recovery from exercise?
- Does Rg1 supplementation influence bone marrow stem cell differentiation in skeletal muscles of older women?
Participants will:
- Pre-test body composition, maximal leg strength, blood and muscle tissue.
- One hour after taking the supplement, participants will perform 4 sets of 10 repetitions of 70% of maximal strength (1RM) leg press and have two thigh muscle biopsies (0 and 24 h after exercise).
- Repeat the protocol one month later on the alternative supplement. Researchers will understand whether Rg1 improves recovery response to resistance exercise in post-menopausal women.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 60 Years 至 70 Years（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •sedentary, able to cease medications and supplements for 48 h before and 24 h after exercise session, medically sound for lower body moderate resistance exercise
排除标准
- •use of insulin, sex hormone and immune-modulating medications, smoking and regular alcohol consumption
研究组 & 干预措施
exercise following Rg1 consumption
1 hour following the consumption of the Rg1 supplement, participants performed exercise intervention before muscle biopsies were taken.
干预措施: exercise following Rg1 consumption (Dietary Supplement)
exercise only following placebo consumption
Crossover trial with placebo supplementation consumed before exercise
干预措施: Placebo (Dietary Supplement)
结局指标
主要结局
Nestin+ neural stem cell area in skeletal muscle biopsies
时间窗: from enrolment to the end of study at 8 weeks
Immunofluorescence staining will be used to semi-quantitatively analyze Nestin+ neural stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
Stro-1+ bone marrow stem cell area in skeletal muscle biopsies
时间窗: from enrolment to the end of study at 8 weeks
Immunofluorescence staining will be used to semi-quantitatively analyze Stro-1+ BMSC presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
CD34+/CD31+ vascular stem cell area in skeletal muscle biopsies
时间窗: from enrolment to the end of study at 8 weeks
Immunofluorescence staining will be used to semi-quantitatively analyzeCD34+/CD31+ vascular stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session.
Serum sex hormone levels 24 h following resistance exercise
时间窗: from enrolment to the end of study at 8 weeks
Forearm blood draw and Elisa analysis of serum for estradiol, progesterone, and testosterone of older women before and 24h after a moderate resistance exercise session.
次要结局
未报告次要终点
研究者
Chia-Hua Kuo
Professor
University of Taipei