Fetuin-A Phosphorylation Status in Insulin Resistance and Metabolic Syndrome
- Conditions
- ObesityMetabolic Syndrome
- Interventions
- Other: Weight loss
- Registration Number
- NCT03478046
- Lead Sponsor
- Auburn University
- Brief Summary
Fetuin-A has been identified as a novel physiological regulator of insulin action in vitro, in intact cells and in vivo in animals. Previous research has shown that circulating levels of fetuin-A were increased in animal models of insulin resistance and diabetes. Additionally, several human investigation studies demonstrate a correlation of fetuin-A levels with body mass index, insulin resistance, and a fatty liver. Recently, the investigators have elucidated the role of fetuin-A phosphorylation in the regulation of insulin action, demonstrating that phosphorylation is critical for the inhibitory activity of fetuin-A. The objectives of this study are twofold: (1) Quantitate phosphorylated fetuin-A levels in individuals with insulin resistance and metabolic syndrome, and (2) Investigate the effects of lifestyle modifications (acute or chronic exercise and dietary modifications) on fetuin-A phosphorylation and insulin sensitivity.
- Detailed Description
There are several objectives of this study: (1) Quantitate phosphorylated fetuin-A levels and the daily variation in these levels in individuals with insulin resistance and metabolic syndrome; (2) Investigate the short-term responses of fetuin-A phosphorylation and changes in insulin resistance that occur with a single session of aerobic exercise; (3) Characterize and compare the total and temporal changes in fetuin-A phosphorylation and insulin resistance that occur with an 8 to 10% weight loss induced by chronic exercise training and dietary modification, and; (4) Investigate the influence of weight loss on the short-term responses of fetuin-A phosphorylation and changes in insulin resistance that occur with a single session of aerobic exercise. Our hypothesis is that phosphofetuin-A levels are tightly correlated with insulin resistance and that lifestyle modifications will improve insulin sensitivity and decrease phosphorylated fetuin-A levels.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 42
- 30 to 65 years of age
- obese (BMI > 30 kg⋅m2 or % fat > 30, and waist girth > 88 cm)
- weight stable over the previous 6 months
- smoker
- documented cardiovascular or metabolic disease
- currently taking medication to alter lipid or glucose metabolism
- practicing regular leisure physical activity over the previous 6 months
- engaged in strenuous vocational activity over the previous 6 months
- signs or symptoms of latent heart disease
- conditions that would preclude treadmill walking
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Obese individuals Weight loss Apparently healthy, weight-stable, obese and physically inactive male volunteers will be recruited. Intervention is an 8 to 10% weight loss induced by chronic exercise training and dietary modification
- Primary Outcome Measures
Name Time Method Total plasma fetuin-A and phosphorylated fetuin-A Total fetuin-A and phosphorylated fetuin-A levels will be measured once every 4 weeks until participant achieves a targeted weight-loss goal of 8-10%. It is anticipated that most participants will achieve their weight-loss goal within 6-10 months. Measure total fetuin-A and phosphorylated fetuin-A levels in plasma
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Auburn University
🇺🇸Auburn, Alabama, United States