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Effect of Treatment With Myo-inositol on Human Semen Parameters in Patients Undergoing In Vitro Fertilization Cycles

Phase 4
Completed
Conditions
Oligoasthenospermia
Sperm Quality
Interventions
Dietary Supplement: myo-inositol + folic acid
Registration Number
NCT01560065
Lead Sponsor
AGUNCO Obstetrics and Gynecology Centre
Brief Summary

Myo-Inositol (MI) is a precursors for the synthesis of phosphatidylinositol polyphosphates (PIPs). In male reproduction, it was shown that MI concentration in the seminiferous tubules is higher than in serum and it was increasing during the movement through the epididymis and the deferent duct. In the present study, the role of myo-inositol has been investigated as a possible antioxidant agent for the systemic treatment of male infertility and the improvement of the sperm's quality.

Samples of seminal fluid were obtained from two groups of patients undergoing to a IVF cycle: healthy subjects and subjects with oligoasthenospermia (OA, \< 15 mil/ml). The collected samples were submitted to optic microscopy in order to evaluate semen's volume, spermatozoa's number and motility, during the initial semen analysis and after density gradient separation method; these parameters were evaluated before and after the administration of 4000mg/die of myo-inositol associated to 400 µg of folic acid for three months.

These values were compared with samples of seminal fluid of healthy patients undergoing to a IVF cycle for a female cause of infertility (control group).

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
Not specified
Inclusion Criteria
  • 3-5 days of sexual abstinence
Exclusion Criteria
  • systemic and endocrine diseases
  • genital infections
  • history of cryptorchidism or varicocele
  • microorchidism
  • hormonal treatment in the last six months
  • alcohol intake, cigarette smoke and/or drug abuse

Study & Design

Study Type
INTERVENTIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Normospermic patientsmyo-inositol + folic acid-
Oligoasthenospermic patientsmyo-inositol + folic acid-
Primary Outcome Measures
NameTimeMethod
Spermatozoa count
Semen volume
Number of spermatozoa after density gradient separation method
Spermatozoa progressive motility
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

University of Catania - Department of Surgery - Section of Obstetrics and Gynecology -Center of Physiopathology of Reproduction

🇮🇹

Catania, Italy

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