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Genomic and Transcriptomic Predictors of Sequential SG Sensitivity After T-DXd in ER+/HER2-Low Metastatic Breast Cancer

Phase 2
Not yet recruiting
Conditions
Breast Cancer
Interventions
Registration Number
NCT06665178
Lead Sponsor
British Columbia Cancer Agency
Brief Summary

Advanced hormone positive (HR+), HER2 negative breast cancer continues to pose a challenge when patients have progressed on CDK4/6 inhibitor and endocrine therapy leaving limited treatment options. Antibody-drug conjugates (ADCs) such as sacituzumab govitecan (SG) and trastuzumab deruxtecan (T-DXd) have changed practice due to significant improvement in progression free survival (PFS) and overall survival (OS) seen in this disease setting. There is a genuine interest to use SG sequentially after T-DXd, however there is no current prospectively curated evidence to support this strategy. Though the epitope is different, the payload are both topoisomerase I inhibitors. Thus, evidence is needed of both clinical efficacy and identification of mechanisms of sensitivity and resistance to sequential ADCs in HER-2 low MBC.

It is hypothesized that performing whole genome and whole transcriptome sequencing in fresh tumour biopsies post progression of T-DXd and prior to SG in ER+/HER2 low metastatic breast cancer (MBC) will provide mechanistic insights into identifying biomarkers, and thus patients, sensitive to sequential SG.

Detailed Description

This is a prospective single-centre Canadian study (BC Cancer Vancouver) enrolling ER+/HER2 low MBC with disease progression after at least one line of endocrine therapy in combination with a CDK 4/6 inhibitor and at least one line of chemotherapy which must include T-DXd as the immediate prior line of treatment in the advanced stage setting.

Patients will receive SG at an initial dose of 10 mg per kilogram intravenously on day 1 and 8 of 21 day cycles. Treatment will continue until evidence of progressive disease, significant toxicity in which patient and or physician wishes to discontinue treatment and/or patient or physician desire to discontinue treatment for any reason.

Tumor specimens will collected from biopsies between the time of informed consent and prior to first administration of SG. The pathology will be reviewed and nucleic acids extracted. Constitutional DNA representing normal cells will be extracted from peripheral blood. PCR-free DNA libraries and either strand-specific or ribo-depleted RNA libraries will be constructed. Following which whole genome sequencing and transcriptome sequencing will be performed.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
20
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Sacituzumab GovitecanSacituzumab GovitecanPatients will receive SG at an initial dose of 10 mg per kilogram intravenously on day 1 and 8 of 21 day cycles.
Primary Outcome Measures
NameTimeMethod
Altered Tumor Genes by PFS Duration on SG Post T-DXdUp to an average of 6 months

Number and identification of altered/mutated genes in the tumors in the subgroup of patients with a longer PFS on SG post T-DXd compared to the tumors in the subgroup of patients with a shorter PFS on SG post T-DXd.

Secondary Outcome Measures
NameTimeMethod
Grade 2-4 toxicitiesUp to an average of 3 months

Number of Participants with the CTCAE version 5 grade 2-4 toxicities reported in SG post T-DXd in ER+/HER2 low MBC

Trop-2 expression and HER-2 expressionUp to an average of 12 months

Trop-2 expression and HER-2 expression by IHC in the metastatic biopsy and correlation with PFS and RR

Dose intensityUp to an average of 3 months

Dose intensity (with and without growth factor support) of SG delivered post T-DXd in ER+/HER2 low MBC

Progression free survival (PFS)Up to an average of 6 months

Progression free survival (PFS) of SG post T-DXd in ER+/HER2 low MBC as measured by RECIST criteria

Response rate (RR)Up to an average of 6 months

Response rate (RR) of SG post T-DXd as measured by RECIST 1.1 criteria

Overall survivalUp to an average of 12 months

Overall survival of SG post T-DXd

Trial Locations

Locations (1)

BC Cancer - Vancouver Center

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Vancouver, British Columbia, Canada

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