Effects of Regular Exercise on Ischemia-Modified Albumin and Total Sulfhydryl Levels in Young Women
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Serum Ischemia-Modified Albumin (IMA)
研究概览
简要总结
Background: Regular physical activity exerts systemic health benefits that extend to redox homeostasis and antioxidant defense mechanisms. Ischemia-modified albumin (IMA) and total sulfhydryl groups (-SH) are increasingly recognized as sensitive biomarkers reflecting oxidative protein modification and thiol-dependent antioxidant capacity, respectively. However, data on their interplay in young women engaged in structured exercise remain scarce.
Objective: This study aimed to investigate the effects of regular exercise on serum IMA and -SH concentrations in healthy young women, thereby providing insights into exercise-induced redox remodeling and sex-specific physiological adaptations.
Methods: A cross-sectional design was employed involving 30 healthy women aged 18-25 years. Participants were divided into an exercise group (n = 15), who had performed structured training ≥3 sessions per week for at least one year, and a control group (n = 15) with no structured exercise history. Venous blood samples were collected after overnight fasting. Serum IMA was quantified using the albumin-cobalt binding assay, and -SH concentrations were determined via the Ellman method. All analyses were performed in duplicate under standardized laboratory conditions. Independent samples t-tests were applied, and effect sizes (Cohen's d) were calculated with 95% confidence intervals.
详细描述
Regular physical activity is widely recognized as a key determinant of human health, conferring benefits across cardiovascular, metabolic, endocrine, and immune systems. Beyond traditional outcomes, habitual exercise plays a pivotal role in regulating oxidative stress and maintaining redox homeostasis. However, few studies have concurrently investigated ischemia-modified albumin (IMA), a marker of oxidative protein modification, and total sulfhydryl groups (-SH), a marker of thiol-dependent antioxidant defense, particularly in young women. The present study was therefore designed to provide novel insights into exercise-induced redox adaptations in this underrepresented population.
This investigation employed an observational, cross-sectional design conducted in accordance with the STROBE guidelines for reporting observational research. Ethical approval was granted by the Muş Alparslan University Scientific Research Ethics Committee (Approval No: 27750; 01 November 2021), and all procedures complied with the Declaration of Helsinki. Written informed consent was obtained from all participants prior to data collection.
A total of 30 healthy women aged 18-25 years were recruited. Participants were categorized into two groups:
Exercise group (n = 15): Women who had been consistently engaged in structured training for at least 12 months, with a minimum frequency of three sessions per week. Training programs were multimodal and typically included aerobic exercise (e.g., running, cycling, swimming at 60-75% HRmax for 20-30 minutes), resistance training (3 sets of 8-12 repetitions at moderate-to-high intensity, targeting major muscle groups), and flexibility exercises incorporated during warm-up and cool-down phases. Training sessions were supervised by qualified instructors in university or private facilities to ensure adherence to safety standards.
Control group (n = 15): Women without a history of structured exercise, whose activity levels were limited to daily living tasks and compulsory school-based physical education.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 25 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Healthy women aged 18-25 years
- •For exercise group: Regular structured training ≥3 sessions per week for at least 12 months
- •For control group: No structured exercise background, only daily living activity
排除标准
- •History of chronic cardiovascular, metabolic, or endocrine disorders
- •Smoking or alcohol abuse
- •Current or recent use (past 6 months) of medications or supplements influencing oxidative stress or hormonal regulation
- •Irregular or intermittent exercise participation not meeting the defined threshold
结局指标
主要结局
Serum Ischemia-Modified Albumin (IMA)
时间窗: Baseline (single fasting blood draw)
Change in serum ischemia-modified albumin (IMA) concentration, measured in absorbance units (ABSU) using the albumin-cobalt binding assay.
次要结局
- Serum Total Sulfhydryl Groups (-SH)(Baseline (single fasting blood draw))
研究者
Omer Faruk Bilici
Principal Investigator
Duvenciler Anadolu Lİsesi