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Effect of Empagliflozin on Liver Fat in Non-diabetic Patients

Phase 4
Completed
Conditions
Non-alcoholic Fatty Liver Disease
Interventions
Drug: Empagliflozin 10 MG
Drug: Placebo pills
Registration Number
NCT04642261
Lead Sponsor
The University of Hong Kong
Brief Summary

Non-alcoholic fatty liver disease (NAFLD) is a global epidemic with a prevalence of 25%. Currently therapies for NAFLD patients without diabetes mellitus (DM) are limited, and are associated with various adverse side effects. Sodium-glucose cotransporter type-2 (SGLT2) inhibitors can reduce hepatic fat content in patients with DM. However, the role of SGLT2 inhibitors in NAFLD patients without DM has not been investigated. Magnetic resonance imaging-derived proton density fat fraction (MRI-PDFF) and liver stiffness measurement (LSM) are non-invasive methods to diagnose hepatic steatosis and fibrosis/cirrhosis, respectively.

The investigators propose a double-blind, randomized, placebo-controlled trial to compare the effects of empagliflozin (a type of SLGT2 inhibitors) versus placebo (in a 1:1 ratio) in reducing hepatic fat content as measured by MRI-PDFF in NAFLD patients without DM. A total of 98 adult patients will be randomly sampled from the liver clinic in our local hospital. Empagliflozin 10mg daily will be given to the treatment arm. The placebo pill will be manufactured to be identical in appearance to the study drug. Eligible subjects will be followed up until week 52, and will undergo clinical, anthropometric and laboratory assessments (including liver function test and fasting blood) at baseline, week 6, 12, 26, 40 and 52. They will undergo LSM at baseline, week 26 and 52, and MRI-PDFF at baseline and week 52. The primary outcome will be a difference in change of liver fat content (measured by MRI-PDFF) at week 52 from baseline between the two groups.

The study results will determine whether SGLT2 inhibitors can reduce hepatic steatosis in NAFLD patients without DM.

Detailed Description

Non-alcoholic fatty liver disease (NAFLD) is a global epidemic with a prevalence of 25%. Currently therapies for NAFLD patients without diabetes mellitus (DM) are limited, and are associated with various adverse side effects. Sodium-glucose cotransporter type-2 (SGLT2) inhibitors are antidiabetic drugs that reduce hepatic fat content in patients with DM, which is independent of glycemic control. However, the role of SGLT2 inhibitors in NAFLD patients without DM has not been investigated. Magnetic resonance imaging-derived proton density fat fraction (MRI-PDFF) is an emerging non-invasive imaging technique, and is more sensitive than liver biopsy/histology in quantifying liver fat change. Liver stiffness measurement (LSM) by transient elastography is a non-invasive method to diagnose fibrosis/cirrhosis with high accuracy.

The novelty of utilizing the concept of "drug repositioning" by changing the role of SGLT2 inhibitors in treating DM to treating NAFLD in patients without DM deserves exploration. The investigators propose a double-blind, randomized, placebo-controlled trial to compare the effects of empagliflozin (a type of SLGT2 inhibitors) versus placebo (in a 1:1 ratio) in reducing hepatic fat content as measured by MRI-PDFF in NAFLD patients without DM. A total of 98 adult patients will be randomly sampled from the liver clinical in our local hospital. Empagliflozin 10mg daily will be given to the treatment arm. The placebo pill will be manufactured to be identical in appearance to the study drug. Eligible subjects will be followed up until week 52, and will undergo clinical, anthropometric and laboratory assessments (including liver function test and fasting blood) at baseline, week 6, 12, 26, 40 and 52. They will undergo LSM at baseline, week 26 and 52, and MRI-PDFF at baseline and week 52. The primary outcome will be a difference in change of liver fat content (measured by MRI-PDFF) at week 52 from baseline between the two groups. The secondary outcomes will be remission of steatosis (MRI-PDFF \<5%) at week 52, reduction of liver fibrosis (LSM) at week 26 and 52, improvement of laboratory results (including liver transaminases and ductal enzymes, fasting glucose, HbA1c, lipid profile), improvement of anthropometric measurements, and combined cardiovascular and cerebrovascular events.

The study results will determine whether SGLT2 inhibitors can reduce hepatic steatosis and regress fibrosis in NAFLD patients without DM.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
98
Inclusion Criteria
  • Potential study subjects will first be screened by transient elastography for the presence of hepatic steatosis (defined as a measurement of controlled attenuation parameter [CAP] >= 248 db/M).
  • They will be recruited into study if steatosis is >= 5% as confirmed by MRI-PDFF
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Exclusion Criteria
  • DM (defined as hemoglobin A1c [HbA1c] >= 6.5% or fasting glucose >= 7.0 mmol/L)
  • alcohol intake > 20g within past 2 years
  • concurrent chronic liver diseases (including chronic viral hepatitis infection, autoimmune hepatitis, Wilson's disease, hemochromatosis, congestive hepatopathy, primary biliary cholangitis, primary sclerosing cholangitis, biliary tract obstruction)
  • drug-induced liver disease
  • usage of drugs that can lead to hepatic steatosis (e.g. steroids, amiodarone, valproate, methotrexate, tamoxifen)
  • decompensated cirrhosis (including ascites, hepatic hydrothorax, variceal bleeding, hepatic encephalopathy, hepatorenal syndrome, hepatopulmonary syndrome)
  • history of malignancy including HCC
  • recreational substance abuse
  • pregnancy
  • contraindications to empagliflozin use (estimated glomerular filtration rate [eGFR] <45mL/min/1.73m2 as measured by the MDRD equation, history of recurrent genitourinary tract infections, gangrene, or allergy)
  • contraindications to MRI (e.g., claustrophobia, certain cardiac pacemakers, implanted medical devices with ferromagnetic properties).
Read More

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Empagliflozin groupEmpagliflozin 10 MGEmpagliflozin 10mg daily for 52 weeks
Placebo groupPlacebo pillsPlacebo pills (identical in appearance to empagliflozin 10mg) daily for 52 weeks
Primary Outcome Measures
NameTimeMethod
Change in liver fat contentweek 52

Difference in the change of liver fat content between the two groups at week 52 from the baseline as measured by MRI-PDFF

Secondary Outcome Measures
NameTimeMethod
Changes of gamma glutamyl transferase (GGT)week 52

Changes of GGT at week 52

Changes of haemoglobin A1c (HbA1c)week 52

Changes of HbA1c at week 52

Changes of total cholesterolweek 52

Changes of total cholesterol at week 52

Changes of waist circumferenceweek 52

Changes of waist circumference at week 52

Remission of steatosisweek 52

Remission of steatosis (defined as MRI-PDFF \< 5%) at week 52

Changes of alanine aminotransferase (ALT)week 52

Changes of ALT at week 52

Changes of alkaline phosphatase (ALP)week 52

Changes of ALP at week 52

Changes of fasting glucoseweek 52

Changes of fasting glucose at week 52

Changes of low density lipoprotein (LDL)week 52

Changes of LDL at week 52

Changes of aspartate aminotransferase (AST)week 52

Changes of AST at week 52

Change of liver fat contentweek 26 and 52

Difference in the change of liver fat content between the two groups at week 26 and 52 from the baseline (CAP measured by transient elastography)

Changes of high density lipoprotein (HDL)week 52

Changes of HDL at week 52

Changes of body weightweek 52

Changes of body weight at week 52

Changes of body mass index (BMI)week 52

Changes of BMI at week 52

Changes of diastolic blood pressureweek 52

Changes of diastolic blood pressure at week 52

Changes of heightweek 52

Changes of height at week 52

Changes of systolic blood pressureweek 52

Changes of systolic blood pressure at week 52

Trial Locations

Locations (1)

The University of Hong Kong/Queen Mary Hospital

🇭🇰

Hong Kong, Hong Kong, China, Hong Kong

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