Investigation of Adipose-Derived Stem Cells in Human Lipoaspirate
Overview
- Phase
- Not Applicable
- Enrollment
- 240
- Locations
- 1
- Primary Endpoint
- Percent of Viable ADSCs
Overview
Brief Summary
The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.
Study Design
- Study Type
- Observational
- Observational Model
- Case Only
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Ages
- 18 Years to 70 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •All adults (18-70 yrs) who have scheduled surgical lipectomy of the hips, abdomen, or thighs are prospective subjects for this study.
Exclusion Criteria
- •Pregnant women will be excluded to protect the unborn child.
Outcomes
Primary Outcomes
Percent of Viable ADSCs
Time Frame: Day of Surgery
Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times.
Secondary Outcomes
- Percent of Apoptotic ADSCs(Day of Surgery)
Investigators
William A. Zamboni, M.D.
Professor and Chair Department of Surgery UNSOM
University of Nevada, Las Vegas