Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 9
- 试验地点
- 1
研究概览
简要总结
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
详细描述
See brief summary for study protocol details
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Sequential
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Being between 18 and 35 years of age
- •Being clinically healthy
- •Actively competing in wrestling
- •Having regular training experience
- •No history of chronic disease
- •No musculoskeletal injury that could affect performance
- •Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period
- •Willing to volunteer and provide written informed consent
排除标准
- •Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- •Having a recent musculoskeletal injury that could affect exercise performance
- •Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- •Having a history of smoking or alcohol abuse
- •Failing to complete the testing procedures and training protocol required for the study
- •Not providing written informed consent
- •Withdrawing voluntarily during the study period
研究组 & 干预措施
Exercise
Participants performed a standardized wrestling exercise session on the first day of testing. Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses.
干预措施: Exercise (Other)
Exercise + Rapid Weight Loss
Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%. On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses.
干预措施: Exercise (Other)
Exercise + Rapid Weight Loss
Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%. On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses.
干预措施: Rapid Weight Loss (Other)