Effect of Omega 3 Fatty Acids on Vascular Function
- Conditions
- Previous Myocardial Infarction
- Registration Number
- NCT01888211
- Lead Sponsor
- University of Edinburgh
- Brief Summary
The mechanisms through which omega-3 fatty acids reduce adverse cardiac events remain uncertain. The aim of the study was to investigate the effect of omega-3 fatty acid supplementation on endothelial vasomotor function, endogenous fibrinolysis, and platelet and monocyte activation in patients with coronary heart disease.
- Detailed Description
Twenty patients with a previous myocardial infarction were recruited into a randomised, double-blind, placebo-controlled, crossover trial of omega-3 fatty acid supplementation (2g/day for 6-weeks). Peripheral blood was taken for analysis of platelet and monocyte activation, and forearm blood flow was assessed in a subset of 12 patients during intrabrachial infusions of acetylcholine, substance P and sodium nitroprusside. Stimulated plasma tissue plasminogen activator (t-PA) concentrations were measured during substance P infusion.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 20
• Myocardial infarction at least 3 months previously.
- Dietary fish allergy or intolerance
- Women of child bearing potential
- Malignant arrhythmias
- Renal or hepatic failure
- Severe or significant co-morbidity
- Previous history of blood dyscrasia
- Unable to tolerate the supine position
- Lack of informed consent
- Blood donation within last 3 months
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Primary Outcome Measures
Name Time Method Endogenous fibrinolysis (net release of plasma t-PA,IU mL-1) Measured at 6 weeks after omega 3 fatty acids or placebo Endogenous fibrinolysis was measured by drawing blood during intrabrachial substance P infusion and then plasma t-PA antigen and activity (t-PA Combi Actibind Elisa Kit; Technoclone, Vienna, Austria) concentrations were determined by enzyme-linked immunosorbent assays. Estimated net release of plasma t-PA was the product of the infused forearm plasma flow (based on the mean hematocrit and the infused forearm blood flow) and the concentration difference between the infused and noninfused arms.
- Secondary Outcome Measures
Name Time Method Endothelial vasomotor function (forearm blood flow, mL l00 mL-1 min-1) Measured at 6 weeks after omega 3 fatty acids or placebo Forearm blood flow was measured during intrabrachial infusions of acetylcholine, substance P and sodium nitroprusside by venous occlusion plethysmography with mercury-in-silicone elastomer strain gauges.
Circulating platelet-monocyte aggregates (%). Measured at 6 weeks after omega 3 fatty acids or placebo Whole blood was immunolabelled with appropriate monoclonal antibodies for subsequent flow cytometric analysis of platelet-monocyte aggregation. Platelet-monocyte aggregates were defined as the percentage of monocytes positive for CD42a.
Trial Locations
- Locations (1)
Centre for Cardiovascular Sciences, University of Edinburgh
🇬🇧Edinburgh, Scotland, United Kingdom
Centre for Cardiovascular Sciences, University of Edinburgh🇬🇧Edinburgh, Scotland, United Kingdom