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Immunological Characteristics of COVID-19 Patients

Conditions
Covid19
Registration Number
NCT04679428
Lead Sponsor
The University Clinic of Pulmonary and Allergic Diseases Golnik
Brief Summary

The study will look at the immunological characteristics of COVID-19 patients and determine neutralizing antibodies against SARS-CoV-2 virus.

Detailed Description

The immunogram (quantitative TBNK immunophenotipisation) will be performed by flow cytometry, the antibody response will be determined by IVD ELISa.

Clinical biochemical and immunochemical (ECLIA) tests (including IL-6) will be performed on Cobas analyzers. Hemogram with differential blood picture will be performed with a hematology analyzer Sysmex XN 3100, examination of blood smears with CellaVision system or. microscope. Coagulation tests will be performed on BCS XP analyzers.

An in-house system for the analysis of SARS-CoV-2 virus fusion inhibitors on human cells will be used to detect the ability of antibodies from patients' serum to neutralize the virus and thus potential protection against re-infection. The system is quantitative, meaning that the titer of such antibodies could be determined, and thus the potential level of protection would be evaluated.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
230
Inclusion Criteria

Provide written consent before being included in the essay.

Positive cases of COVID-19 (based on polymerase chain reaction or positive antigen test for SARS-CoV-2).

Exclusion Criteria

Patients who are unable to give consent or who are unable to follow up will be excluded.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Immunological changes during and after COVID-19 infectionJuly 2022

Correlation between TBNK dynamic and increase of neutralising antibodies

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

University Clinic of Respiratory and Allergic Diseases Golnik

🇸🇮

Golnik, Slovenia

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