Evaluation of The Effect of 980nm Diode Laser Intracanal Irradiation on Levels of Interleukin-8 Expression
- Conditions
- Endodontically Treated Teeth
- Interventions
- Other: PlaceboDevice: Intracanal Diode laser irradiation
- Registration Number
- NCT05603988
- Lead Sponsor
- Cairo University
- Brief Summary
This clinical trial will be conducted to evaluate the amount of Il-8 expression before and after the application of intracanal irradiation with diode laser 980 nm versus placebo in failed endodontic cases with periapical lesions.
- Detailed Description
* Thorough medical and dental history for the eligible patients
* Thorough intraoral and extraoral diagnosis
* Local anesthesia
* Isolation and removal of any previous filling or restoration
* Removal of old gutta percha and establish patency to the full working length.
* 1st sample: will be collected using 3 sterile paper points
* Chemo-mechanical preparation.
* 2nd sample collection
• Laser group: The canals will be irradiated with a high-power diode laser, with wavelength of 980 nm, maximum output of 3 watts and coupled with a fiber optic tip of 200 µm diameter.
• Mock laser application (Placebo group): The fiberoptic tip will be inserted inside the root canal, mimicking the laser irradiation, but not activated.
* 3rd sample collection
* A piece of dry cotton will be placed in the access cavity, and then will be sealed with glass ionomer filling material
* 2nd visit: 1 week later, 4th sample collection then Obturation
* Number of visits \& follow up period: Two visits
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 28
- failed previously treated endodontic single-rooted teeth with chronic periapical lesions; with radiographic evidence of periapical radiolucency of not less than 1 cm diameter will be selected.
- Teeth with straight root canals.
- No contributory medical history.
- No previous administration of analgesics and/or antibiotics within the previous 2 weeks.
- Retreatment cases with complications such as separated instruments, perforations, ledges, resorption, transportation, calcifications, immature apices and over fillings.
- Teeth with curved roots.
- Swelling or sinus tract.
- Mutilated teeth that interfere with proper isolation and seal between visits.
- Subjects with generalized periodontitis, or if the tooth has a probing depth of more than 3 mm.
- Subjects with uncontrolled diabetes or debilitating diseases.
- Pregnant or nursing females.
- Systemic disorders that necessitate antibiotic prophylaxis or prevent the use of Ibuprofen.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Placebo group Placebo • Mock laser application (Placebo group): The fiber optic tip will be inserted inside the root canal, mimicking the laser irradiation group, but not activated Diode Laser group Intracanal Diode laser irradiation Intracanal Diode laser application
- Primary Outcome Measures
Name Time Method quantification of IL-8 one week - After collection of all the samples, quantification of IL-8 will be determined using ELISA kit before (S-1), after chemo-mechanical preparation (S-2), after intracanal irradiation or placebo effect (S-3) and last after one week of the treatment (S-4). The kit is used according to the manufacturer's recommendations.
- Secondary Outcome Measures
Name Time Method Postoperative pain 6, 12, 24 hours and 7 days Following the first visit, the modified visual analogue scale (VAS scale) with 11 points where 0 means no pain and 10 means maximum pain will be used to record patient's level of pain
Trial Locations
- Locations (1)
Faculty of Dentistry
🇪🇬Cairo, Egypt