Analysis of T Cell Metabolism in Acute Myeloid Leukemia Patients
- Conditions
- Acute Myeloid Leukemia
- Registration Number
- NCT04259372
- Lead Sponsor
- University of Freiburg
- Brief Summary
The objective of this study was to analyze the T cell metabolism and immune phenotype in AML patients during the course of the disease before and after allo-HCT.
- Detailed Description
Patients who experience relapse of acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (allo-HCT) have a dismal prognosis. Infusion of donor T cells can induce graft-versus-leukemia (GVL) effects, indicating the importance of intact T cell function for leukemia control. Recent studies have shown the importance of metabolic fitness for an effective T cell response.
In this study we have analyzed the T cell metabolism and immune phenotype in AML patients at different time points before and after allo-HCT.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 77
- confirmed diagnosis of AML
- age ≥ 18 years
- peripheral blood sample available
- written informed consent
- ability to understand the nature of the study and the study related procedures and to comply with them
- age < 18 years
- lack of informed consent
- patients that cannot be classified in one of the 3 groups
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method T cell metabolism 4 years Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) determined by live-cell metabolic assay using the Seahorse Analyzer
T cell phenotype 4 years T cell cytokine and effector molecule production
- Secondary Outcome Measures
Name Time Method Metabolite abundance in serum 4 years Serum metabolites as measured by mass spectrometry