Vaginal Microbiome Influence on the Results of Assisted Reproduction Techniques

Not Applicable

Completed

• Conditions: Implantation Failure
• Interventions: Other: Probiotic

• Registration Number: NCT03153787
• Lead Sponsor: Instituto Bernabeu

Brief Summary

The objective of the present study to analyze the vaginal microbiome in patients who have not achieved gestation after oocyte donation treatment and after probiotic administration.

All patients will receive vaginal probiotic according to the standard regimen. Sample collection will be performed the embryo transfer day and on the day of the pregnancy test. Follow up of gestation will be carried out.

The analysis of the vaginal microbiome will be performed though massive genetic sequencing (determination of 16SRNA).

The study variables, among others, are vaginal microbiome pattern after probiotic administration, the gestation rate, and the abortion rate.

Detailed Description

This study will be performed after the observational study "Vaginal microbiome influence on the results of assisted reproduction techniques". The main goal of this previous study will be to identify patterns of vaginal and / or endometrial microbiome in relation to the gestation rate in women with implantation failure and / or repeat abortions who undergo an oocyte donation treatment. The vaginal and endometrial microbiome of women with (i) implantation failure, (ii) repeat abortions and (iii) control group without implantation failure or repeat abortions, will be analyzed before and after the embryo transfer.

Those women who do not achieve pregnancy will be eligible for the present study. The objective of the present study to analyze the vaginal microbiome in patients who have not achieved gestation after oocyte donation treatment and after probiotic administration.

All patients will receive vaginal probiotic according to the standard regimen: 1 tablet (via vaginal) for 5 days after menstruation during the same cycle of the next embryo transfer.

The vaginal sample will be analyzed after taking the probiotic. Sample collection will be performed the embryo transfer day and on the day of the pregnancy test. Follow up of gestation will be carried out.

Vaginal sample collection: obtention of vaginal fluid with vaginal swab by direct visualization. Direct visualization means having the patient in a lithotomy position, like a routine gynecological examination by visualization with a vaginal speculum.

The analysis of the vaginal microbiome will be performed though massive genetic sequencing (determination of 16SRNA). First of all, the extraction of RNA and subsequent analysis. We will analyze the groups of microorganisms, as well as the determination of patterns related to failure of implantation, repetitive abortions and gestation success.

The study variables, among others, are vaginal microbiome pattern after probiotic administration, the gestation rate, and the abortion rate. The vaginal microbiome will be compared with the results of the previous observational study.

Study Timeline

• Study First Submitted: 2017-05-10
• Study First Submitted QC: 2017-05-12
• Study First Posted: 2017-05-15
• Study Start: 2017-06-01
• Primary Completion: 2019-05-01
• Study Completion: 2019-05-01
• Status Verified: 2022-03
• Last Update Submitted: 2022-03-30
• Last Update Posted: 2022-03-31

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 69

Inclusion Criteria

• Treated by oocyte donation.
• Transfer of a single euploid embryo in the blastocyst phase.
• <3 months prior without antibiotic treatment.
• Women who have completed the previous observational study "Study of the influence of vaginal and endometrial microbiome on the results of assisted reproduction techniques".
• Women who have not achieved gestation after treatment.
• Signed informed consent form.

Exclusion Criteria

• Uterine malformations.
• Not treated hydrosalpinx
• Known factors of implantation failure and repetitive abortions, such as haematological disorders of hypercoagulability.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Not pregnancy	Probiotic	Vaginal probiotic administration

Primary Outcome Measures

Name	Time	Method
Change from Baseline Vaginal microbiome at 2 and 4 weeks	2 weeks and 4 weeks	Vaginal microbiome will be analyzed through massive genetic sequencing (determination of 16S RRNA) Before and after probiotic administration
Gestation rate	4 weeks	Gestation rate after assisted reproduction technique
Abortion rate	45 weeks	Abortion rate (clinical and biochemical) after assisted reproduction technique

Secondary Outcome Measures

Name	Time	Method
Live newborn rate	45 weeks	Live newborn rate after assisted reproduction technique
Complications during gestation	45 weeks	Complications during gestation after assisted reproduction technique

Trial Locations

Locations (1)

Instituto Bernabeu; 🇪🇸 Alicante, Spain