Effects of Whey Protein and Collagen Supplementation

Not Applicable

Completed

• Conditions: Exercise; Muscle Strength; Nutrition Therapy; Hypertrophy; Resistance Training
• Interventions: Dietary Supplement: Whey protein supplementation; Dietary Supplement: Collagen supplementation

• Registration Number: NCT04213586
• Lead Sponsor: Universidade Norte do Paraná

Brief Summary

The present project aims to investigate the effects of high-quality protein (whey protein) vs. low-quality protein (collagen) on muscle mass and function (e.g., strength and power) during a 10-wk resistance training program in young adults. It will be tested the hypothesis that leucine-matched collagen should promote similar gains on muscle function and mass compared to whey protein supplementation.

Detailed Description

The purpose of this study will be to investigate the effects of whey protein vs. leucine-matched collagen supplementation on muscle mass and function after a 10-wk resistance training program. Initially, all participants will be submitted for a 2-wk familiarization period, and then randomized into two groups (N = 11 / group): whey protein group and leucine-matched collagen group. The following variables will be assessed from pre-to post-training: anthropometric, muscle thickness from vastus lateralis and biceps brachii muscles using ultrasound, training load, mean power and peak torque for unilateral arm flexion exercise using a isokinetic dynamometer, countermovement vertical jump, nutritional intake, and testosterone-to-cortisol ratio . Shapiro-Wilk and Levine tests will be used to test the normality and homogeneity of the measurements, respectively. Two-way ANOVA (group x time) tests for repeated measures will be performed to assess changes over time and between groups for all dependent variables. Violation of sphericity will adjust using Greenhouse- Geyser correction. The Bonferroni post-hoc test will detect specific differences between groups. When appropriate, the percentage of change (∆%) will be reported according to the following equation: ∆% = \[(pre-average post average) / pre-average mean\] × 100. Values will be express as mean ± standard deviation. O significance level α will be 0.05.

Study Timeline

• Study Start: 2019-09-01
• Primary Completion: 2019-11-01
• Study Completion: 2019-12-05
• Study First Submitted: 2019-12-11
• Study First Submitted QC: 2019-12-27
• Study First Posted: 2019-12-30
• Status Verified: 2020-01
• Last Update Submitted: 2020-01-28
• Last Update Posted: 2020-01-30

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 22

Inclusion Criteria

• Men and women aged 18-35 years
• To be eutrophic (not overweight or obese)

Exclusion Criteria

• To present a history of musculoskeletal disorders
• Be attending a systematized weight training program for at least six months
• To make use of medicines that could affect muscle function.
• To have used ergogenic supplements and anabolic steroids for at least six months before study
• To have restrictions for exercises practice considering Physical Activity Readiness Questionnaire (PAR-Q) responses.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Whey Protein	Whey protein supplementation	Each participant will be supplemented with whey protein (7 d/wk) during 10 weeks.
Leucine-matched collagen	Collagen supplementation	Each participant will be supplemented with collagen (7 d/wk) during 10 weeks.

Primary Outcome Measures

Name	Time	Method
Change in biceps brachii thickness	Baseline and after 10 weeks	Measured by ultrasonography pre- and post-training
Change in vastus lateralis thickness	Baseline and after 10 weeks	Measured by ultrasonography pre- and post-training
Change in vertical jump	Baseline and after 10 weeks	Countermovement vertical jump performance will be assessed using the Smart Jump instrument
Change in mean power	Baseline and after 10 weeks	Mean power using a isokinetic dynamometer (BIODEX)
Change in peak torque	Baseline and after 10 weeks	Peak torque using a isokinetic dynamometer (BIODEX)
Change in muscle strength by training load	Baseline, 5 and 10 weeks	Training load for leg press, knee extension, and arm flexion exercises

Secondary Outcome Measures

Name	Time	Method
Change in testosterone-to-cortisol ratio	Baseline and after 10 weeks	Blood samples will be collected for analyse of the testosterone-to-cortisol ratio
Dietary intake	Baseline, 2, 5, 10 weeks	Dietary intake to be assessed using 3-day diet recalls.

Trial Locations

Locations (1)

Universidade Norte do Paraná; 🇧🇷 Londrina, Paraná, Brazil