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A Study of JNJ-73763989, Pegylated Interferon Alpha-2a and Nucleos(t)Ide Analogs in Participants With Chronic Hepatitis B Virus Infection

Phase 2
Terminated
Conditions
Hepatitis B, Chronic
Interventions
Drug: JNJ-73763989
Drug: PegIFN-alpha-2a
Drug: Tenofovir disoproxil
Drug: TAF
Drug: ETV
Registration Number
NCT05005507
Lead Sponsor
Janssen Research & Development, LLC
Brief Summary

The purpose of this study is to evaluate the efficacy in terms of hepatitis B surface antigen (HBsAg) changes from baseline for the treatment regimens of 24 weeks of JNJ-73763989 + 24 weeks of nucleos(t)ide analog (NA) + 12 or 24 weeks of pegylated interferon alpha-2a (PegIFN-alpha-2a) (with immediate or delayed start of PegIFN-alpha-2a treatment).

Detailed Description

JNJ-73763989 (JNJ-3989) is a liver-targeted antiviral therapeutic for subcutaneous injection designed to treat chronic hepatitis B virus (HBV) infection via a ribonucleic acid interference (RNAi) mechanism. Combination treatment with JNJ-73763989 and NA has the potential to specifically decrease HBV viral antigen levels and inhibit viral replication. Since HBsAg is immune suppressive, the direct reduction of HBsAg levels by JNJ-73763989 is anticipated to contribute to the restoration of the immune response that is impaired in chronic HBV infection. Pegylated interferon (PegIFN) is an approved drug for the treatment of chronic HBV infection and after a finite treatment duration of 48 weeks results in slightly increased HBsAg seroclearance rates. The primary hypothesis of this study is that at least one of the combination regimens of JNJ-73763989+NA+PegIFN-alpha-2a is more efficacious than NA treatment alone (standard of care), as measured by the primary efficacy endpoint. This study will be conducted in 3 periods: Screening Period (4 weeks), Treatment Period (24 weeks) and Follow-up (FU) Period (48 weeks), starting at Week 24. Safety assessments will include adverse events (AEs), serious AEs, clinical safety laboratory tests, electrocardiograms (ECGs), vital signs, ophthalmologic examinations and physical examinations. Total duration of individual participation will be up to 76 weeks (including screening period).

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
1
Inclusion Criteria
  • Medically stable based on physical examination, medical history, vital signs, and 12-lead electrocardiogram (ECG) performed at screening
  • Participants must have a body mass index between 18.0 and 35.0 kilograms per meter square (kg/m^2) inclusive
  • Participants with chronic hepatitis B who should: a) be chronic hepatitis B e antigen (HBeAg) -negative; b) be anti-HBe antibody-positive; c) be currently receiving nucleos(t)ide analog (NA) treatment for at least 2 years prior to screening; d) have serum hepatitis B virus (HBV) deoxyribonucleic acid (DNA) less than (<) 60 international unit/milliliter (IU/mL) on 2 sequential measurements at least 6 months apart; e) have alanine aminotransferase (ALT) values < 2.0x upper limit of normal (ULN) on 2 sequential measurements at least 6 months apart
  • Hepatitis B surface antigen (HBsAg) greater than (>) 5 IU/mL at screening
  • Fibroscan liver stiffness measurement less than or equal to (<=) 9.0 kilopascal (kPa) within 6 months prior to screening
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Exclusion Criteria
  • History or signs of cirrhosis or portal hypertension
  • Evidence of hepatitis A, C, D, E virus infection, or human immunodeficiency virus (HIV) infection
  • Liver disease of non-HBV etiology
  • Clinically relevant alcohol or drug abuse within 12 months of screening
  • Participants who meet any of the additional exclusion criteria for pegylated interferon alpha-2a (PegIFN- α2a) as described in local prescribing information (example, refer to Pegasys SmPC or Pegasys USPI) per the investigator's discretion. Key exclusion criteria for PegIFN- α2a include: a) Participants with signs or symptoms compatible with autoimmune disorders. b) Participants with bone marrow suppression. c) Participants with hypoglycaemia, hyperglycaemia, and/or diabetes mellitus, who cannot be effectively controlled by medication. d) Participants with pre-existing ophthalmologic disorders. e) Participants with one or more of the following laboratory abnormalities: i) Absolute neutrophil count less than (<)1,500 cells/mm3 (<1,000 cells/mm³ for black or African American participants). ii) Serum creatinine >1.5x ULN. iii) Inadequately controlled thyroid function (thyroid stimulating hormone [TSH] and thyroxine [T4]). f) Participants with a history of a severe psychiatric disorder including severe depression, suicidal ideation and attempted suicide, or a current depression or other psychiatric disorder that is not adequately controlled on a stable medication regimen
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Arm 3: JNJ-73763989 + NA + PegIFN-alpha-2aJNJ-73763989Participants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from baseline till Week 12.
Arm 1: JNJ-73763989 + nucleos(t)ide analog (NA) + pegylated interferon alpha-2a (PegIFN-alpha-2a)JNJ-73763989Participants will receive JNJ-73763989 subcutaneous (SC) injection once every 4 weeks for 24 weeks plus NA treatment (either entecavir \[ETV\], tenofovir disoproxil or tenofovir alafenamide \[TAF\] tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly for 24 weeks.
Arm 1: JNJ-73763989 + nucleos(t)ide analog (NA) + pegylated interferon alpha-2a (PegIFN-alpha-2a)PegIFN-alpha-2aParticipants will receive JNJ-73763989 subcutaneous (SC) injection once every 4 weeks for 24 weeks plus NA treatment (either entecavir \[ETV\], tenofovir disoproxil or tenofovir alafenamide \[TAF\] tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly for 24 weeks.
Arm 1: JNJ-73763989 + nucleos(t)ide analog (NA) + pegylated interferon alpha-2a (PegIFN-alpha-2a)TAFParticipants will receive JNJ-73763989 subcutaneous (SC) injection once every 4 weeks for 24 weeks plus NA treatment (either entecavir \[ETV\], tenofovir disoproxil or tenofovir alafenamide \[TAF\] tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly for 24 weeks.
Arm 1: JNJ-73763989 + nucleos(t)ide analog (NA) + pegylated interferon alpha-2a (PegIFN-alpha-2a)ETVParticipants will receive JNJ-73763989 subcutaneous (SC) injection once every 4 weeks for 24 weeks plus NA treatment (either entecavir \[ETV\], tenofovir disoproxil or tenofovir alafenamide \[TAF\] tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly for 24 weeks.
Arm 2: JNJ-73763989 + NA + PegIFN-alpha-2aJNJ-73763989Participants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil, or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from Week 12 till Week 24.
Arm 2: JNJ-73763989 + NA + PegIFN-alpha-2aPegIFN-alpha-2aParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil, or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from Week 12 till Week 24.
Arm 2: JNJ-73763989 + NA + PegIFN-alpha-2aTAFParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil, or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from Week 12 till Week 24.
Arm 2: JNJ-73763989 + NA + PegIFN-alpha-2aETVParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil, or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from Week 12 till Week 24.
Arm 3: JNJ-73763989 + NA + PegIFN-alpha-2aPegIFN-alpha-2aParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from baseline till Week 12.
Arm 3: JNJ-73763989 + NA + PegIFN-alpha-2aTAFParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from baseline till Week 12.
Arm 3: JNJ-73763989 + NA + PegIFN-alpha-2aETVParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from baseline till Week 12.
Arm 1: JNJ-73763989 + nucleos(t)ide analog (NA) + pegylated interferon alpha-2a (PegIFN-alpha-2a)Tenofovir disoproxilParticipants will receive JNJ-73763989 subcutaneous (SC) injection once every 4 weeks for 24 weeks plus NA treatment (either entecavir \[ETV\], tenofovir disoproxil or tenofovir alafenamide \[TAF\] tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly for 24 weeks.
Arm 2: JNJ-73763989 + NA + PegIFN-alpha-2aTenofovir disoproxilParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil, or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from Week 12 till Week 24.
Arm 3: JNJ-73763989 + NA + PegIFN-alpha-2aTenofovir disoproxilParticipants will receive JNJ-73763989 SC injection once every 4 weeks for 24 weeks plus NA treatment (either ETV, tenofovir disoproxil or TAF tablets orally) once daily for 24 weeks plus PegIFN-alpha-2a SC injection once weekly from baseline till Week 12.
Primary Outcome Measures
NameTimeMethod
Percentage of Participants With a Reduction of at Least 2log10 International Units Per Milliliter (IU/mL) in Hepatitis B Surface Antigen (HBsAg) Levels From Baseline at Week 24 (End of Study Intervention [EOSI])Week 24

Percentage of participants with a reduction of at least 2log10 IU/mL in HBsAg levels from baseline at Week 24 (EOSI) were planned to be reported.

Secondary Outcome Measures
NameTimeMethod
Percentage of Participants With Adverse Events (AEs)Up to 1 month 26 days

An AE was any untoward medical occurrence in a participant who received study drug without regard to possibility of causal relationship.

Percentage of Participants With Serious Adverse Events (SAEs)Up to 1 month 26 days

An AE was any untoward medical occurrence in a participant who received study drug without regard to possibility of causal relationship. SAE is any untoward medical occurrence that at any dose may result in death, is life-threatening, requires inpatient hospitalization or prolongation of existing hospitalization, results in persistent or significant disability/incapacity, is a congenital anomaly/birth defect, is a suspected transmission of any infectious agent via a medicinal product.

Percentage of Participants With Abnormalities in Clinical Laboratory TestsUp to 1 month 26 days

Percentage of participants with abnormalities in clinical laboratory tests including hematology, blood coagulation, blood biochemistry, urinalysis, urine chemistry, renal biomarkers, were reported.

Percentage of Participants With Abnormalities in 12-Lead Electrocardiograms (ECGs)Up to 1 month 26 days

Percentage of participants with abnormalities in 12-Lead ECGs were reported.

Percentage of Participants With Abnormalities in Vital SignsUp to 1 month 26 days

Percentage of participants with abnormalities in vital signs were reported.

Percentage of Participants With HBsAg Seroclearance at Follow-up Weeks 24 and 48 Without Re-starting NA TreatmentFollow-up Weeks 24 and 48

Percentage of participants with HBsAg seroclearance at follow-up Weeks 24 and 48 without re-starting NA treatment were planned to be reported. Seroclearance of HBsAg is defined as a (quantitative) HBsAg level \<lower limit of quantitation (LLOQ). LLOQ is 0.05 International Units per milliliter (IU/mL).

Percentage of Participants With Hepatitis B Virus (HBV) Deoxyribonucleic Acid (DNA) < (Less Than) LLOQ at Follow-up Weeks 24 and 48 Without Re-starting NA TreatmentFollow-up Weeks 24 and 48

Percentage of participants with HBV DNA \<LLOQ at follow-up Weeks 24 and 48 without re-starting NA treatment were planned to be reported. LLOQ is 0.05 IU/mL.

Change From Baseline in HBsAg Over TimeBaseline up to Week 72

Change from baseline in HBsAg over time were planned to be reported.

Time to Achieve HBsAg SeroclearanceUp to 72 weeks

Time to achieve HBsAg seroclearance were planned to be reported. Time to achieve HBsAg seroclearance is defined as the number of days between the date of first study intervention intake and the date of the first occurrence of HBsAg seroclearance (that is, the date of the first HBsAg seroclearance - the date of first study intervention intake + 1). Seroclearance of HBsAg is defined as a (quantitative) HBsAg level \<LLOQ. LLOQ is 0.05 IU/mL.

Time to Achieve HBsAg SeroconversionUp to 72 weeks

Time to achieve HBsAg seroconversion were planned to be reported. Time to achieve HBsAg seroconversion is defined as the number of days between the date of first study intervention intake and the date of the first occurrence of HBsAg seroclearance and appearance of anti-HBs antibodies (that is, the date of the first HBsAg seroclearance and anti-HBs antibodies - the date of first study intervention intake + 1). Seroconversion of HBsAg is defined as having achieved HBsAg seroclearance (quantitative HBsAg level \<LLOQ) and appearance of anti-HBs antibodies. LLOQ is 0.05 IU/mL.

Time to Achieve HBV DNA <Lower Limit of Quantitation (LLOQ)Up to 72 weeks

Time to achieve HBV DNA \<LLOQ were planned to be reported. Time to achieve HBV DNA \<LLOQ is defined as the number of days between HBV DNA \>LLOQ for participants who re-treated with NA and the date of the first occurrence of HBV DNA \< LLOQ after NA re-treatment (that is, the date of the HBV DNA \< LLOQ after being re-treated with NA - the date of first occurrence of HBV DNA\>LLOQ + 1). LLOQ is 0.05 IU/mL.

Percentage of Participants With Abnormalities in Ophthalmologic ExaminationWeeks 8 and 20

Percentage of participants with abnormalities in ophthalmologic examination were planned to be reported.

Percentage of Participants Meeting the Protocol-defined Nucleos(t)Ide Analog (NA) Treatment Completion Criteria Based on the Week 24 (EOSI) or Follow-up Week 2 VisitsWeek 24 (EOSI) and follow-up Week 2

Percentage of participants meeting the protocol-defined NA treatment completion criteria based on the Week 24 (EOSI) or follow-up Week 2 visits was planned to be reported. NA treatment completion criteria were as follows: (a) participant had alanine transaminase (ALT) \<3\*upper limits of normal (ULN); (b) participant had Hepatitis B Virus (HBV) Deoxyribonucleic acid (DNA) \<20 international units per milliliter (IU/mL); (c) participant was Hepatitis B e antigen (HBeAg)-negative; (d) participant had Hepatitis B surface antigen (HBsAg\<10 IU/mL.

Percentage of Participants With Abnormalities in Physical ExaminationWeek 24

Percentage of participants with abnormalities in physical examination were planned to be reported.

Percentage of Participants With Virologic FlaresUp to 1 month 26 days

Percentage of participants with virologic flares were reported. The start of a confirmed virologic flare is defined as the first date of two consecutive visits with HBV DNA \>200 IU/mL. The end date of the same confirmed virologic flare is defined as the first date when HBV DNA value returns to less than or equal to (\<=)200 IU/mL or the date of NA treatment restart, whichever comes first.

Percentage of Participants With Biochemical FlaresUp to 1 month 26 days

Percentage of participants with biochemical flares were reported. The start date of a confirmed off-treatment biochemical flare: the first date of two consecutive visits with ALT and/or AST\>=3x ULN and \>=3x off-treatment/on-treatment nadir (that is, lowest value observed during off-treatment period up to the time point of meeting the biochemical flare criteria) while the participant does not receive any of the study interventions. The end date of the same off-treatment/on-treatment biochemical flare: the first date when there is a 50% reduction from the peak ALT and/or AST level \& \<3x ULN.

Percentage of Participants Requiring Nucleos(t)Ide Analog (NA) Re-treatmentUp to 72 weeks

Percentage of participants requiring NA re-treatment were planned to be reported.

Percentage of Participants With HBsAg, Hepatitis B e Antigen (HBeAg), HBV DNA, and Alanine Aminotransferase (ALT) Levels Below/Above Different Cut-offsUp to 72 weeks

Percentage of participants with HBsAg, HBeAg, HBV DNA, and ALT levels below/above different cut-offs were planned to be reported.

Percentage of Participants With HBsAg SeroconversionUp to 72 weeks

Percentage of participants with HBsAg seroconversion were planned to be reported. Seroconversion of HBsAg is defined as having achieved HBsAg seroclearance (quantitative HBsAg level \<LLOQ) and appearance of anti-HBs antibodies. LLOQ is 0.05 IU/mL.

Percentage of Participants With Virologic BreakthroughUp to Week 24

Percentage of participants with virologic breakthrough were planned to be reported. HBV virological breakthrough is defined as having a confirmed on-treatment HBV DNA increase by \>1 log10 from nadir level (lowest level reached during treatment) in participants who did not have on-treatment HBV DNA level below the LLOQ or a confirmed on-treatment HBV DNA level \>200 IU/mL in participants who had on-treatment HBV DNA level below the LLOQ. LLOQ is 0.05 IU/mL.

Serum Concentration of JNJ-73763989 (JNJ-73763924 and JNJ-73763976)Up to 72 weeks

Serum concentration of JNJ-73763989 (JNJ-73763924 and JNJ-73763976) were planned to be reported.

Serum Concentration of Nucleos(t)Ide Analog (NA) (Entecavir [ETV])Up to 72 weeks

Serum concentration of NA (ETV) was planned to be reported.

Serum Concentration of PegIFN-alpha-2aUp to 72 weeks

Serum concentration of PegIFN-alpha-2a was planned to be reported.

Trial Locations

Locations (12)

I.D. Care, Inc.

🇺🇸

Hillsborough, New Jersey, United States

Hosp. Univ. Vall D Hebron

🇪🇸

Barcelona, Spain

Hosp. Univ. Infanta Leonor

🇪🇸

Madrid, Spain

Hosp. Alvaro Cunqueiro

🇪🇸

Vigo, Spain

GI Research Institute (G.I.R.I.)

🇨🇦

Vancouver, British Columbia, Canada

ID Clinic

🇵🇱

Mysłowice, Poland

Kagawa Prefectural Central Hospital

🇯🇵

Takamatsu, Japan

EMC Instytut Medyczny SA

🇵🇱

Wroclaw, Poland

Hosp. Univ. Marques de Valdecilla

🇪🇸

Santander, Spain

National Cheng Kung University Hospital

🇨🇳

Tainan, Taiwan

PUNKT ZDROWIA Hlebowicz Jakubowski Lekarze sp.p.

🇵🇱

Gdansk, Poland

Vancouver ID Research and Care Centre Society

🇨🇦

Vancouver, British Columbia, Canada

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