Microarchitecture of Vitreoretinal Contact

Not Applicable

Completed

• Conditions: Vitreoretinal Detachment; Vitreo-Retinal Adhesion
• Interventions: Other: scanning electron microscopy

• Registration Number: NCT04802889
• Lead Sponsor: The S.N. Fyodorov Eye Microsurgery State Institution

Brief Summary

Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.

Detailed Description

Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion.

Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation.

Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous.

Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous.

Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.

Study Timeline

• Study Start: 2008-01-01
• Primary Completion: 2020-01-01
• Study First Submitted: 2020-12-06
• Study Completion: 2020-12-31
• Status Verified: 2021-03
• Study First Submitted QC: 2021-03-15
• Last Update Submitted: 2021-03-15
• Study First Posted: 2021-03-17
• Last Update Posted: 2021-03-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 20

Inclusion Criteria

• eyes from donors without concomitant multiple organ pathology and ophthalmic pathology

Exclusion Criteria

• eye injury, age over 45 years, concomitant multiple organ pathology, previous eye diseases

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
cadaver eyes	scanning electron microscopy	The Central region of the retina and preretinal structures of cadaver eyes are examined

Primary Outcome Measures

Name	Time	Method
the ILM thickness in the macular zone (foveola)	within 3 days after receiving the cadaver eyes	the ILM thickness in the macular zone (foveola) in mkm by scanning electron microscopy data
superficial microstructure of ILM	within 3 days after receiving the cadaver eyes	by scanning electron microscopy data
features of the structural microarchitecture of the preretinal vitreous in the macular zone	within 3 days after receiving the cadaver eyes	by scanning electron microscopy data
morphofunctional features of preretinal hyalocytes.	within 3 days after receiving the cadaver eyes	by scanning electron microscopy data

Trial Locations

Locations (1)

S.N. Fedorov Nmrc "Mntk "Eye Microsurgery"; 🇷🇺 Moscow, Russian Federation