Identification of the Cutaneous Microbiota in Patients With Cutaneous Infection Admitted in the Department of Acute Infectious Diseases
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Skin Infections
- Sponsor
- Assistance Publique Hopitaux De Marseille
- Enrollment
- 100
- Locations
- 1
- Primary Endpoint
- Distribution of microorganisms found according to patient characteristics
- Last Updated
- 6 years ago
Overview
Brief Summary
Identify the cutaneous microbiota on a cutaneous lesion (cellulite, wound, rash, etc.) on a swab, biopsies or abscess puncture and on "healthy" skin on a skin swab performed for cutaneous mapping to search for staphylococcal deposits.
Detailed Description
Cellulite is characterized by inflammation and an alleged infection of the skin and subcutaneous tissues. Previous studies have shown a low yield of samples such as biopsy and needle aspiration of the order of 16%. The most common pathogens are Staphylococcus aureus and Streptococcus pyogenes. Conventional cultivation has limitations such as the lack of detection of fastidious microorganisms, or the use of antibiotics. Molecular diagnostic methods, such as detection of 16S ribosomal DNA followed by amplification and pyrosequencing, have been used to overcome the limitations of microbial culture. In a recent study, the authors investigated the causes of acute cellulitis without performing drainage but skin biopsies from the infected site and another non-infected site by quantitative PCR, pyrosequencing, and conventional culture. PCR identified methicillin-resistant S. aureus methicillin in approximately 30% -40% of cases with similar frequency in infected and uninfected sites. In another study R. felis was found in not only bedsores, but also, swabs taken from healthy skin, as well as Streptococcus pneumoniae, S. aureus and Streptococcus group A. Being engaged in the exploration of the human microbiota especially by culture we propose to extend the knowledge of this skin microbiota in patients hospitalized in Infectious Disease services.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Patient with skin infections at admission
- •Patient requiring skin mapping
- •Person informed of the study and having expressed no opposition to participate in the study.
- •Affiliate or beneficiary of a social security
Exclusion Criteria
- •Subject not agreeing to participate
- •Vulnerable person: pregnant, parturient or nursing woman, person under guardianship or curatorship, or deprived of liberty by a judicial or administrative decision.
Outcomes
Primary Outcomes
Distribution of microorganisms found according to patient characteristics
Time Frame: 36 moths
Diagnosis by culture with identification by "Maldi tof" on cutaneous samples Molecular diagnosis with specific PCR identification of pathogens: Staphylococcus aureus, Streptococcus A, 16S rRNA and sequencing,