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Dose-Ranging Study of Sofosbuvir in Combination With Pegylated Interferon and Ribavirin in Treatment Naïve GT 1 HCV Patients

Phase 2
Completed
Conditions
Hepatitis C
Interventions
Registration Number
NCT01054729
Lead Sponsor
Gilead Sciences
Brief Summary

Participants with genotype 1 HCV infection were randomized to 1 of 3 sofosbuvir doses (100 mg, 200 mg, or 400 mg) or matching placebo once daily based upon stratification for IL28B status (CC or CT/TT). Placebo tablets were administered to participants receiving 100 mg active sofosbuvir (3 placebo tablets) and 200 mg active sofosbuvir (2 placebo tablets) in order to maintain the study blind. Participants received sofosbuvir/matching placebo from Day 0 to 27. Participants also received treatment with PEG+RBV starting on Day 0 of the study which continued for 48 weeks. Participants were evaluated for sustained virologic response (SVR) for an additional 24 weeks following completion of study treatment.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
64
Inclusion Criteria
  • Treatment-naive males and females, 18-65 years of age
  • Genotype 1 HCV infection
  • Negative pregnancy test for females of childbearing age
  • Females of childbearing age and males with female partners of childbearing age must use two forms of contraception during treatment and following the last dose of ribavirin in accordance with locally approved label for ribavirin
Exclusion Criteria
  • Hepatitis B or HIV infection
  • Pregnant or breast feeding females or male partners of pregnant females
  • Previous interferon or ribavirin-based therapy or investigational anti-HCV agent
  • History or evidence of medical condition associated with chronic liver disease other than HCV

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Sofosbuvir 100 mg+PEG+RBVSofosbuvirParticipants received sofosbuvir 100 mg (1 x 100 mg tablet) and placebo to match sofosbuvir (3 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 100 mg+PEG+RBVPEGParticipants received sofosbuvir 100 mg (1 x 100 mg tablet) and placebo to match sofosbuvir (3 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 100 mg+PEG+RBVRBVParticipants received sofosbuvir 100 mg (1 x 100 mg tablet) and placebo to match sofosbuvir (3 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 200 mg+PEG+RBVPEGParticipants received sofosbuvir 200 mg (2 x 100 mg tablets) and placebo to match sofosbuvir (2 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 200 mg+PEG+RBVRBVParticipants received sofosbuvir 200 mg (2 x 100 mg tablets) and placebo to match sofosbuvir (2 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 400 mg+PEG+RBVSofosbuvirParticipants received sofosbuvir 400 mg (4 x 100 mg tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 400 mg+PEG+RBVPEGParticipants received sofosbuvir 400 mg (4 x 100 mg tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 400 mg+PEG+RBVRBVParticipants received sofosbuvir 400 mg (4 x 100 mg tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Placebo+PEG+RBVPlaceboParticipants received placebo to match sofosbuvir (4 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Placebo+PEG+RBVPEGParticipants received placebo to match sofosbuvir (4 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Placebo+PEG+RBVRBVParticipants received placebo to match sofosbuvir (4 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Sofosbuvir 200 mg+PEG+RBVSofosbuvirParticipants received sofosbuvir 200 mg (2 x 100 mg tablets) and placebo to match sofosbuvir (2 tablets) for 28 days (baseline to Day 28), plus PEG+RBV (baseline to Week 48)
Primary Outcome Measures
NameTimeMethod
Percentage of Participants Who Experienced Adverse Events During the Sofosbuvir Treatment PeriodBaseline to Week 4

Adverse events (AEs) occurring during the sofosbuvir treatment period were summarized across the participant population. A participant was counted once if they had a qualifying event.

Secondary Outcome Measures
NameTimeMethod
Change in Circulating HCV RNA at Week 4Baseline to Week 4
Percentage of Participants With Sustained Virologic Response (SVR) at 12 and 24 Weeks After Last Dose of PEG+RBV Following Completion of 48 Weeks of TreatmentPost-treatment Weeks 12 and 24

SVR at 12 weeks (SVR12) and 24 weeks (SVR24) was defined as HCV RNA \< LOD 12 and 24 weeks after last dose of PEG+RBV, respectively, following completion of 48 weeks of treatment (4 weeks of sofosbuvir or matching placebo and PEG+RBV, followed by an additional 44 weeks of PEG+RBV).

Plasma Pharmacokinetics of Sofosbuvir: Cmax at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The Cmax of sofosbuvir was measured at Day 27 following continuous dosing of sofosbuvir.

Plasma Pharmacokinetics of GS-331007: AUCtau at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The AUCtau of GS-331007 was analyzed at Day 27 (following continuous dosing of sofosbuvir).

Plasma Pharmacokinetics of GS-566500: Cmax at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The Cmax of GS-566500 was measured at Day 0 following a single dose of sofosbuvir. GS-566500 is one of the major metabolites of sofosbuvir.

Plasma Pharmacokinetics of GS-566500: Cmax at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The Cmax of GS-566500 was measured at Day 27 following continuous dosing of sofosbuvir.

Plasma Pharmacokinetics of GS-566500: AUCinf at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The AUCinf of GS-566500 was analyzed at Day 0 (following a single dose of sofosbuvir).

Plasma Pharmacokinetics of GS-566500: AUCtau at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The AUCtau of GS-566500 was analyzed at Day 27 (following continuous dosing of sofosbuvir).

Percentage of Participants Who Developed Resistance to SofosbuvirBaseline to Week 4
Percentage of Participants With Rapid Virologic Response at Week 4Week 4

Rapid virologic response (RVR) was defined as HCV RNA below the limit of detection (LOD \[15 IU/mL\]) at Week 4.

Plasma Pharmacokinetics of Sofosbuvir: Cmax at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The Cmax of sofosbuvir was measured at Day 0 following a single dose of sofosbuvir.

Cmax is defined as the maximum concentration of drug.

Plasma Pharmacokinetics of Sofosbuvir: AUCinf at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The AUCinf of sofosbuvir was analyzed at Day 0 (following a single dose of sofosbuvir).

AUCinf is defined as the concentration of drug (area under the plasma concentration versus time curve) extrapolated to infinite time.

Plasma Pharmacokinetics of Sofosbuvir: AUCtau at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The AUCtau of sofosbuvir was analyzed at Day 27 (following continuous dosing of sofosbuvir).

AUCtau is defined as the concentration of drug (area under the plasma concentration versus time curve) over the dosing interval.

Plasma Pharmacokinetics of GS-331007: Cmax at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The Cmax of GS-331007 was measured at Day 0 following a single dose of sofosbuvir. GS-331007 is the predominant circulating metabolite of sofosbuvir.

Plasma Pharmacokinetics of GS-331007: Cmax at Day 27Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, and 24 hours postdose)

The Cmax of GS-331007 was measured at Day 27 following continuous dosing of sofosbuvir.

Plasma Pharmacokinetics of GS-331007: AUCinf at Day 0Predose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, and 12 hours postdose

The AUCinf of GS-331007 was analyzed at Day 0 (following a single dose of sofosbuvir).

Trial Locations

Locations (7)

Duke University

🇺🇸

Durham, North Carolina, United States

Alamo Medical Research Center

🇺🇸

San Antonio, Texas, United States

Virginia Mason Medical Center

🇺🇸

Seattle, Washington, United States

Fundacion de Investigacion de Diego

🇵🇷

Santurce, Puerto Rico

Quest Clinical Research

🇺🇸

San Francisco, California, United States

University of Florida

🇺🇸

Gainesville, Florida, United States

Orlando Immunology Center

🇺🇸

Orlando, Florida, United States

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