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Safety of Various Mode of Delivery of Iron Supplement on Iron Toxicity Markers in Preschool Children

Phase 3
Conditions
Iron Overload
Oxidative Stress
Interventions
Dietary Supplement: Iron Supplementation
Registration Number
NCT00980421
Lead Sponsor
Annamalai University
Brief Summary

The purpose of study is to evaluate and compare the effect of iron supplementation when given as iron tablet or fortified biscuit or combined with zinc in the form of tablet on morbidity and iron toxicity markers among children aged 24-36 months.

Detailed Description

The study will be carried out in two phases in Sangam Vihar, Delhi. The survey will be conducted to identify children in the age group of 24-36 months. After obtaining consent, an enrollment form with detailed socio-demographic information about the child will be filled. Blood and urine samples will be collected for estimation of iron status, oxidative stress, immune and interleukin markers. Based on iron status, two randomization lists will be generated i.e. one for iron sufficient and other for iron deficient children. Children will be randomly allocated to receive one of the four interventions (60 in each group) for 180 days. The intervention will be either Iron Tablets (IT) or Iron and Zinc tablets (IZ) or Biscuits fortified with Iron (IB), or Placebo tablets (CO). Children in the iron groups (IT and IB) will receive 12.5 mg/day of iron. Children allocated to receive Iron and Zinc (IZ group) will receive 10 mg/day of Zinc in addition to 12.5 mg/day of Iron. The fortified biscuits (IB) will be formulated by CFTRI, Mysore, India and will have iron in the form of iron sulphate. The iron (IT group) or iron and zinc (IZ group) fortified dispersible tablets will be procured from WHO manufactured by Nutriset (Maluanlay, France). Tablets will have iron salt in the form of ferrous sulphate and zinc in the form of zinc sulphate. The interventions at the baseline on Day 3, on Day 30 (on completion of Phase -I) and on Day 180 (on completion of Phase-II) will be given at the clinic prior to the post 3 hour blood collection. Regular supplementation will be given at home. Children will be followed up on a biweekly basis by the field worker for delivery of the intervention, collection of morbidity and compliance information from the mother/caretaker of the child. During follow-up, blood sample for interleukin levels will be taken in the event of an illness such as diarrhea, pneumonia, fever and severe illness/hospitalization. At the completion of Phase-I (on day 30) and Phase - II (on day 180), the blood and urine samples will be collected again for estimation of iron status, oxidative stress, immune and interleukin markers.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
240
Inclusion Criteria
  • between 24-36 months of age
  • not severely malnourished or ill requiring hospitalization
  • willing to stay in the study area for 6 months
  • consent to participate
Exclusion Criteria
  • not consented
  • severely malnourished or ill requiring hospitalization

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
ITIron SupplementationIron Tablet group (12.5 mg/d) + Placebo Biscuit
IZIron SupplementationIron (12.5mg/d)+Zinc (10 mg/d) Tablet Group + Placebo Biscuit
IBIron SupplementationIron Fortified Biscuit Group(12.5 mg/d)+ Placebo Tablet
COIron SupplementationPlacebo Tablet + Placebo Biscuit
Primary Outcome Measures
NameTimeMethod
The effect of intervention on immune response, iron over load, oxidative stress markers and iron status0 month, 1 month and 6 months
Secondary Outcome Measures
NameTimeMethod
Primary Outcome Measures: Mortality Incidence of disease requiring hospitalization interleukin response during severe illness or hospitalization180 days

Trial Locations

Locations (1)

Center for Micronutrient Research

🇮🇳

Delhi, India

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