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Exenatide Once Weekly, Cardiovascular Risk and Type-2 Diabetes

Phase 4
Completed
Conditions
Type 2 Diabetes Mellitus
Interventions
Registration Number
NCT02380521
Lead Sponsor
University of Palermo
Brief Summary

The glucagon-like peptide 1 (GLP-1) agonist exenatide represents an effective therapy in patients with type 2 diabetes mellitus (T2DM), which also seems to have some important non-glycemic effects. Yet, these non-glycemic effects are still largely unknown.

The effect of exenatide once weekly was investigated in controlled, blinded and open-label clinical studies in subjects with T2DM who were controlled on diet and exercise alone or in combination with oral antidiabetic agents, but also in multi-dose controlled studies and such studies resulted in significant reductions in glycemic parameters (mean glycated hemoglobin (HbA1c), fasting serum/plasma glucose as well as postprandial plasma glucose levels), but also in body weight, over 24 to 30 weeks. Meaningful reductions were observed as early as week 4 of treatment, and maintained through 6 years of treatment.

The study investigating cardiovascular effects of exenatide once weekly is currently undergoing. The results available are not numerous (such as DURATION-2, DURATION-3, DURATION-4 studies) and cannot lead to definitive conclusions.

In this study the investigators will evaluate the effect of exenatide once-weekly on multiple cardiovascular risk markers. These markers are related to subclinical atherosclerosis, endothelial dysfunction, oxidative stress and atherogenic lipoproteins.

The investigators will perform an open label, single-arm, prospective, intervention study using exenatide once weekly for a period of 8 months on 60 patients with T2DM.

Detailed Description

The research hypothesis is to assess whether exenatide once weekly can reduce subclinical atherosclerosis (as measured by carotid-intima media thickness \[cIMT\]) in patients with T2DM.

The primary objective is to assess whether exenatide once weekly can reduce subclinical atherosclerosis (as measured by cIMT) in patients with T2DM.

Primary endpoint: Reduction in cIMT. The secondary objective is to assess whether exenatide once weekly can reduce atherogenic lipoproteins, oxidative stress and endothelial dysfunction in patients with T2DM.

Secondary endpoint: Reduction in atherogenic lipoproteins, oxidative stress and improvement of endothelial dysfunction.

The purpose of the present 8 months study is to elucidate largely unknown non-glycemic effects of exenatide as add-on to metformin, including effects on cIMT, oxidative stress parameters and atherogenic lipoproteins, in addition to its benefit on glycemic control, weight loss and other efficacy parameters, as well as the safety profile of exenatide.

The data for clinical and biochemical analyses, including collection of efficacy variables, ECG, pulse and physical examination, will be collected at baseline and after 8 months of therapy (at 6 months of the treatment, only the routine laboratory analyses, including HbA1c, will be performed).

Clinical diagnostic tools will include the measurement of:

1. cIMT, that will be assessed by B-mode real-time ultrasound using a single sonographer (Medison SonoAce Pico, with a probe of 7.5-10.0 MHz) in a standardized manner with fixed angles of insonation;

2. Endothelial dysfunction, that will be assessed by flow mediated dilation of brachial artery.

Biochemical analyses will include the analysis of:

1. Routine testing: blood testing, liver and kidney profile, plasma lipids, glucose metabolism parameters;

2. Oxidative stress parameters (plasma glutathione, serum lipid hydroperoxides, reactive oxygen species);

3. Atherogenic lipoproteins, e.g. the analysis of 11 distinct lipoproteins including very-low-density lipoprotein (VLDL), 3 intermediate density lipoprotein (IDL) subclasses and 7 low density lipoprotein (LDL) subclasses.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
60
Inclusion Criteria
  1. Provision of informed consent prior to any study specific procedures;
  2. Men and women with T2DM aged >18 years;
  3. BMI >25 kg/m2;
  4. HbA1c 7.5-8.5 %;
  5. Receiving metformin therapy (doses ranging from 1500 to 3000 mg daily) for at least 8 weeks.
Exclusion Criteria
  1. Pregnancy or willingness to become pregnant;
  2. Moderate and severe liver dysfunction;
  3. Moderate and severe renal failure;
  4. Previous major cardiovascular event;
  5. Severe infections at the discretion of the investigator (such as human immunodeficiency virus [HIV], hepatitis B virus [HBV] and hepatitis C virus [HCV]);
  6. Any malignancy;
  7. Plasma triglycerides >400 mg/dL, plasma LDL-cholesterol > 250 mg/dL.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
60 patients with T2DMExenatide once weekly (BYDUREON™)These patients will be treated with exenatide once weekly for a period of 8 months
Primary Outcome Measures
NameTimeMethod
Subclinical atherosclerosis (as measured by cIMT) in patients with T2DM treated with exenatide once weeklyChange from baseline to 8 months of the treatment

Reduction in cIMT after 8 months of the treatment

Secondary Outcome Measures
NameTimeMethod
Atherogenic lipoproteins (as measured by the analysis of 11 distinct lipoprotein subclasses using the Lipoprint system®) in patients with T2DM treated with exenatide once weeklyChange from baseline to 8 months of the treatment

Reduction in atherogenic lipoproteins including small dense LDL particles after 8 months of the treatment

Oxidative stress (as measured by markers of oxidative stress) in patients with T2DM treated with exenatide once weeklyChange from baseline to 8 months of the treatment

Reduction in oxidative stress after 8 months of the treatment

Endothelial dysfunction (as measured by flow mediated dilation of brachial artery) in patients with T2DM treated with exenatide once weeklyChange from baseline to 8 months of the treatment

Improvement of endothelial dysfunction as indicated by changes in flow mediated dilation of brachial artery after 8 months of the treatment

Trial Locations

Locations (1)

University Hospital of Palermo

🇮🇹

Palermo, Italy

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