Effect of Exercise on MiR-126 in Individuals With Prediabetes: A Randomized Controlled Trial
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 64
- Locations
- 2
- Primary Endpoint
- Delta CT mir-126
Study Overview
Brief Summary
This study aims to investigate the effect of a structured exercise program on microRNA-126 (miR-126) expression in individuals with prediabetes.
Detailed Description
This study aims to investigate the effect of a structured exercise program on microRNA-126 (miR-126) expression in individuals with prediabetes. The hypothesis of our study is that regular aerobic exercise will increase miR-126 expression. Determining the effect of exercise on miR-126 in individuals with prediabetes will contribute to the elucidation of the pathways through which exercise is effective in prediabetes via epigenetic mechanisms.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Prevention
- Masking
- Double (Care Provider, Outcomes Assessor)
Eligibility Criteria
- Ages
- 18 Years to 60 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Individuals who were newly diagnosed with prediabetes according to the Oral Glucose Tolerance Test (OGTT) and/or HbA1C criteria
- •between the ages of 18-60
- •were physically inactive (Level 1 according to the International Physical Activity Questionnaire)
- •were motivated to exercise
- •Individuals who had no obstacle to exercise as a result of the evaluation made by a cardiologist (ECG, exercise ECG test, etc.)
- •and who did not show a change in body weight of more than ±2.5 kg in the last six months were included in the study.
Exclusion Criteria
- •presence of serious medical conditions (e.g. advanced cancer, major neurological or endocrine disorders, respiratory failure)
- •current cardiovascular disease, BMI <19 kg/m²
- •history of metformin use within the last 6 months
- •receiving hormone replacement therapy
- •life expectancy less than one year
- •HIV positivity
- •substance use
- •or orthopedic and cognitive disorders that may prevent exercise
Outcomes
Primary Outcomes
Delta CT mir-126
Time Frame: 1 week
Ct (cycle threshold) values for miR-126 and miR-16-5p were used for analysis. ΔCt and ΔΔCt values were calculated as follows, and expression levels were analyzed using the 2-ΔΔCt method: ∆Ct = Ct (gene of interest) - Ct (housekeeping gene) ΔΔCt = ∆Ct (treated sample) - ∆Ct (untreated sample) Higher ΔCt values indicate lower expression levels.
Secondary Outcomes
- BMI(1 day)
Investigators
Elif Yıldırım Ayaz
Assoc. Prof. Dr.
Sultan Abdulhamid Han Training and Research Hospital, Istanbul, Turkey