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Effect of chronic kidney disease on leukocyte functio

Conditions
N18.3
N18.4
N18.5
Chronic kidney disease, stage 3
Chronic kidney disease, stage 4
Chronic kidney disease, stage 5
Registration Number
DRKS00005794
Lead Sponsor
niversitätsklinikum Münster
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Pending
Sex
All
Target Recruitment
80
Inclusion Criteria

CKD patients in stage 3-5, with last dialysis >48h

Exclusion Criteria

1 Age <18 years
2 pregnancy
3 Immunosuppressive therapy (within the last 7 days)
4 Acute kidney injury
5 Dialysis in the past 48 hours
6 Major hematologic pre-existing conditions
7 Known HIV infection.
8 acute or chronic inflammation
9 State after organ transplantation
10 Known autoimmune disease

Study & Design

Study Type
observational
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Rolling velocity and arrest of leukocyte in ex vivo flow chamber experiments
Secondary Outcome Measures
NameTimeMethod
The influence of chronic uremia on the intracellular signal transduction in activated leukocytes is investigated by biochemical assays (western blot). Expression of adhesion molecules on the surface of isolated neutrophils is analyzed by flow cytometry. Demographic and clinical data are collected and analyzed in order to accurately describe the disease severity and progression of chronic kidney injury (laboratory data, general infectious monitoring, monitoring of renal replacement therapy).<br>
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