Alcohol Induced de Novo Lipogenesis in Women
- Conditions
- Liver Metabolism
- Registration Number
- NCT04829110
- Lead Sponsor
- University of Texas Southwestern Medical Center
- Brief Summary
The study is cross-sectional is design. In the first Aim of this study, 5 women and 5 men will be asked to consume 2g/kg of \[2H\]water (a.k.a. deuterium oxide or heavy water) which incorporates 2H tracers into newly synthesized fatty acids and triglycerides. Deuterated water is not radioactive and has a long history of application in human studies. Researchers will collect blood samples 2 hours before and 5 hours after the participants consume 20 grams of alcohol as vodka to measure alcohol induced hepatic de novo lipogenesis (DNL) in both men in women. In addition, for Aim 2 researchers will recruit an additional 10 women who will be randomized into one of two groups who will consume a beverage containing vodka and sucrose, or sucrose alone. Aim 2 will be identical to the experimental scheme in Aim 2 in order to determine if sucrose enhances the effects of vodka on hepatic DNL.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ACTIVE_NOT_RECRUITING
- Sex
- All
- Target Recruitment
- 27
- Male or Female
- Ages 21 - 45
- Healthy
- Light to moderate alcohol consumption (≤1 drink a day (~14g) in women and ≤2 drinks a day (~28 g) in men)
- BMI between 18.5-29.9
- Normal Nutritional Status
- Ability to speak and understand English
- BMI >29.9
- Comorbid conditions
- Any regular medications, except Oral Contraceptives
- Pregnant or Breastfeeding
- Regular exercise above activities of daily living
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Mean Change in Hepatic De Novo Lipogenesis (DNL) 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, and 7 hours post heavy water consumption DNL will be measured by Orbitrap high-resolution-mass-spectrometry (GC-Orbitrap-HRMS)
We will measure changes in the incorporation of 2H tracers into plasma triglycerides over time. This data will be analyzed by averaging each time point and looking at change in de novo lipogenesis over time.
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
School of Health Professions at UTSW
🇺🇸Dallas, Texas, United States
School of Health Professions at UTSW🇺🇸Dallas, Texas, United States