Quercetin in Coronary Artery By-pass Surgery
- Conditions
- Coronary Artery Disease
- Interventions
- Drug: Placebo
- Registration Number
- NCT04907253
- Lead Sponsor
- Montreal Heart Institute
- Brief Summary
The purpose of this study is to test the anti-inflammatory and anti-senescence effects of quercetin during coronary artery by-pass graft surgery.
- Detailed Description
After being informed about the study and the potential risk, all patients giving written informed consent will be randomized in a double-blind manner (participant and investigators) on 1:1 ratio to receive quercetin (500 mg twice daily) or placebo (twice daily) starting 2 days before a coronary artery by-pass graft surgery and for the duration of their hospitalization but up to 10 days (i.e. up to 7 days post-surgery). Blood (5 ml) will be collected the first morning after recruitment (t-1), 24h post-surgery (t1), day 4 post-surgery (t2) and day of hospital discharge for blood analyses. During the surgery, if a discarded segment of mammary artery is available, it will be collected for laboratory work. Health status will be assessed during the follow-up visit 8 to 12 weeks post-surgery.
Recruitment & Eligibility
- Status
- ACTIVE_NOT_RECRUITING
- Sex
- All
- Target Recruitment
- 100
- to be able to speak French or English;
- to be able to give free and enlighten consent;
- be hospitalized and waiting for a cardiac surgery of revascularization;
- to have had a myocardial infarction (MI) within the past 30 days or to be in a state of stable angina before the surgery.
- to be in a stable state without MI in the last 30 days;
- have a cardiac surgery concomitant to the cardiac surgery of revascularization;
- have an infection in the last 30 days;
- to have renal insufficiency (GFR less than 30);
- to have a liver disease (AST, ALT or bilirubin ˃ 2X normal values);
- to have a known cirrhosis;
- to have a past history of breast cancer or other tumors estrogen-dependent;
- to be intolerant to flavonoids, niacine or ascorbic acid;
- take quinolone;
- need for a quinolone during post-op;
- not being able to give a free and enlighten consent;
- not being able to speak French of English;
- take quercetin as a supplement.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Placebo Placebo Patients receiving placebo twice daily Quercetin Quercetin Patients receiving 500 mg quercetin twice daily
- Primary Outcome Measures
Name Time Method Quercetin-associated change in surgery-associated marker of senescence Baseline, 1 day and 4 days post-surgery Circulating blood levels of ANGPTL2 (ng/L) will be measured.
Quercetin-associated change in surgery-associated inflammation Baseline, 1 day and 4 days post-surgery Circulating blood levels of hs-CRP (mg/L) will be measured.
- Secondary Outcome Measures
Name Time Method Quercetin-dependent change in endothelium-dependent relaxation During surgery When available, a discarded segment of the mammary artery will be collected at the end of the cardiac surgery.
One portion of the segment will be used to study the endothelium-dependent relaxation ex vivo. Segments will be mounted on a wire myograph. After an equilibration period, it will be pre-contracted with U46619 (0.1 µM): when the pre-contraction is stable, a cumulative dose-response curve to acetylcholine is built to induce an endothelium-dependent relaxation. Relaxation will be determined by two parameters, the vascular sensitivity to acetylcholine (µM) and the maximal relaxation induced by acetylcholine (% of relaxation).Quercetin-dependent change in senescent endothelial cell load During surgery When available, a discarded segment of the mammary artery will be collected at the end of the cardiac surgery.
One portion of the segment will be used to isolate arterial wall cell nuclei for single nuclei mRNA sequencing. Senescence is assessed by expression levels of p21 mRNA, p16 mRNA and ANGPTL2 mRNA.Quercetin-dependent change in senescent arterial wall cell load During surgery When available, a discarded segment of the mammary artery will be collected at the end of the cardiac surgery.
One portion of the segment will be stored at -80oC for bulk mRNA sequencing. Arterial wall senescence will be assessed by expression levels of p21 mRNA, p16 mRNA and ANGPTL2 mRNA.
Trial Locations
- Locations (1)
Montreal Heart Institute
🇨🇦Montréal, Quebec, Canada