The Effect of Dietary Nitrate on the Oral Microbiota, Markers of Nitric Oxide Bioavailability and Cardiovascular Health in in Young and Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 101
- 试验地点
- 1
- 主要终点
- Quantity of nitrate -reducing bacteria
研究概览
简要总结
This study evaluates whether dietary manipulation of the oral microbiota modulates cardiovascular health through effects on nitric oxide bioavailability.
详细描述
Nitric oxide is an important molecule for human health because it controls blood flow and blood pressure. With aging, our ability to make nitric oxide gets worse and our blood pressure tends to increase. We can help the body produce more nitric oxide by supplementing the diet with vegetables, such as beetroot juice, which contains high amounts of nitrate. This has been shown to improve blood flow and blood pressure. The bacteria inside our mouths play an important role in helping convert the ingested nitrate to nitric oxide. The reason for this study is to find out whether there are differences in oral bacteria between young and older adults, and how the oral bacterial community and blood flow and blood pressure might change when diet is supplemented with natural, nitrate-rich beetroot juice and when we use antibacterial mouthwash. This study will help us understand how the bacteria inside our mouths might be useful for keeping us healthy.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy young (18-30 yr) and older adults (65-80 yr)
- •Male and female
- •Willing to undergo the experiments as detailed in the informed consent documentation
- •Willing and capable of providing written, informed consent.
排除标准
- •Individuals receiving medication for pulmonary, cardiovascular, or metabolic conditions, ulcerative colitis or renal disease
- •Having an active oral disease or dentures
- •Having resting BP >140/90 mmHg
- •Having used antibiotics within 3 months
- •Lacking willingness or capacity to give informed consent.
研究组 & 干预措施
Nitrate-rich beetroot juice
2 x 70mL concentrated juice per day for 14 days
干预措施: Nitrate-rich beetroot juice (Dietary Supplement)
Nitrate-depleted beetroot juice
2 x 70mL concentrated juice per day for 14 days
干预措施: Nitrate-depleted beetroot juice (Dietary Supplement)
Antibacterial mouthwash
2 x 10mL antibacterial mouthwash per day for 14 days
干预措施: Mouthwash (Other)
结局指标
主要结局
Quantity of nitrate -reducing bacteria
时间窗: 14 days
Change in total quantity of nitrate-reducing bacteria as measured by sequencing bacterial 16S rRNA genes
次要结局
- Nitric oxide bioavailability - salivary nitrite(14 days)
- Nitric oxide bioavailability - plasma nitrate(14 days)
- Blood pressure(14 days)
- Nitric oxide bioavailability - salivary nitrate(14 days)
- Flow mediated dilation(14 days)
- Nitric oxide bioavailability - plasma nitrite(14 days)