Metabolic Adaptations to Chronic and Acute Exercise in Overweight Adults (ATX-Study)
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Michigan
- Enrollment
- 60
- Locations
- 1
- Primary Endpoint
- PPARg mRNA expression
Study Overview
Brief Summary
The primary purpose of this study is to examine the effects of chronic exercise training and an acute session of exercise on key risk factors associated with Metabolic Syndrome (e.g., glucose tolerance, blood lipid profile, and blood pressure) and alterations in subcutaneous adipose tissue structure and metabolic function in overweight adults.
Detailed Description
Subjects will be asked to complete the Substrate Metabolism Laboratory General Screening Questionnaire and American Heart Association Health/Fitness Questionnaire to determine if they are eligible to complete the preliminary testing procedures. If eligible, subjects will be asked to visit the Substrate Metabolism Laboratory to participate in the preliminary testing procedures (listed below) before participating in the experimental trial.
PRELIMINARY TESTING PROCEDURES:
- Blood pressure, height and weight measurements
- Body Composition Assessment (~20 min)
- Exercise Fitness Test (~10 min)
- Resting Metabolic Rate (RMR) measurement (~30 min)
- 3 hour Oral Glucose Tolerance Test (OGTT)
EXPERIMENTAL TRIAL:
The evening before the experimental trial, subjects will eat a standardized dinner meal and snack. The meal and snack must be eaten at specific times as indicated by the research staff. After eating the snack, subjects will need to fast (no food or beverages - besides water) overnight.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 40 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Age: 18-40
- •Body Mass Index: 25-35 kg/m2
- •Regular exercisers: (≥4 days/wk of aerobic exercise; 30-60min/session at moderate and vigorous intensities)
- •Non-exercisers: no regularly planned exercise/physical activity
- •Women must have regularly occurring menses and must be premenopausal
Exclusion Criteria
- •Blood pressure > 140/90 mm Hg
- •Pregnant or lactating
- •Evidence/history of cardiovascular or metabolic disease
- •Medications known to affect lipid or glucose metabolism
Arms & Interventions
Exercise session
Subjects will exercise at a moderate intensity for 60 minutes. There are no different arms in this study.
Intervention: Exercise session (Other)
Outcomes
Primary Outcomes
PPARg mRNA expression
Time Frame: change from baseline at 1 hour post exercise
The study team will quantify the abundance of key adipogenic markers in adipose tissue biopsy samples
Cytokine abundance in adipose tissue
Time Frame: change from baseline at 1 hour post exercise
The study team will measure cytokine proteins in adipose tissue
Secondary Outcomes
- Total Cholesterol(change from baseline at 1 hour post exercise)
Investigators
Jeffrey F Horowitz
Professor, Movement Science; Director, Substrate Metabolism Laboratory
University of Michigan