Quantitative and qualitative determination of natural occuring regulatory t cells in blood and bronchoalveolar lavage fluid of patients with pulmonary sarcoidosis and healthy volunteers.

Completed

• Conditions: Besnier Boeck disease; Sarcoidosis; 10006436

• Registration Number: NL-OMON29919
• Lead Sponsor: St. Antonius Ziekenhuis

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 24 June 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 70

Inclusion Criteria

Clinical strong suspicion of sarcoidosis
Alveolar lymphocytosis (>=15%)
Histological confirmed sarcoidosis
firstly presenting

Exclusion Criteria

previous steroid use , smoking

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>phenotypical analysis: 1 - expression of protein (absolute en percentual)<br /><br>2 - intensity of protein expression per<br /><br>cel<br /><br><br /><br>both in lavage-fluid and peripheral blood<br /><br><br /><br>functional analysis: 1 - suppression of proliferation (%)<br /><br>2 - cytokine measurement (concentration)<br /><br>in peripheral blood<br /><br><br /><br>physiological analysis: measurement of auto-antibodies in peripheral blood<br /><br><br /><br>genetic analysis: when abovementiones analyses show significant differences<br /><br>with respect to protein expression or cytokine production relevant genes<br /><br>(PTPN22, CTLA4, CD103, TGF&beta;-1) will be genotyped and screened for present<br /><br>polymorphisms.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>not applicable</p><br>