Diurnal Variability in the Regulation of Beta-cell Function and Insulin Sensitivity in Overweight People
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 16
- 试验地点
- 1
- 主要终点
- Determine postprandial hepatic insulin sensitivity (suppression of endogenous glucose production) after ingesting breakfast and dinner meals.
研究概览
简要总结
The purpose of this research study is to learn more about how our body produces sugar, breaks down fat for fuel, and makes insulin (the major hormone that controls the production of blood sugar and fat breakdown) during a 24-hour day and how body fat and muscle are involved in these processes.
详细描述
The purpose of this study is to determine whether there are diurnal differences in postprandial beta-cell function and hepatic insulin sensitivity and the factors that influence these metabolic functions, including insulin signaling, adipose tissue and systemic inflammation, nicotinamide phosphoribosyltransferase (NAMPT)-mediated nicotinamide adenine dinucleotide(NAD) biosynthesis, and sirtuin (silent mating type information regulation 2 homolog 1 (SIRT1)) in overweight human subjects.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •18-55 years old
- •BMI between 25.0-29.9 kg/m2
- •Must be sedentary (regular exercise <1hour/week or <2 times/week
排除标准
- •Regular exercise (>1hour/week or >2 times/week)
- •Severe organ dysfunction
- •Severe hypertriglyceridemia (>300 mg/dl)
- •Medications that may alter the results of the study
- •Breastfeeding
结局指标
主要结局
Determine postprandial hepatic insulin sensitivity (suppression of endogenous glucose production) after ingesting breakfast and dinner meals.
时间窗: 24 hours
Postprandial pancreatic hepatic insulin sensitivity will be evaluated by using a mixed meal labelled with stable isotope tracers, in conjunction with stable isotope tracer infusion. Metabolic outcomes from the breakfast meal will be compared with values obtained after dinner.
Determine postprandial beta-cell function (insulin secretion) after ingesting breakfast and dinner meals.
时间窗: 24 hours
Postprandial pancreatic beta-cell function will be evaluated by using a mixed meal labelled with stable isotope tracers, in conjunction with stable isotope tracer infusion. Metabolic outcomes from the breakfast meal will be compared with values obtained after dinner.
次要结局
- Determine whether there is diurnal variability in adipose tissue and systemic inflammation.(24 hours)
- Determine whether there is diurnal variability in NAMPT-mediated NAD+ biosynthesis and SIRT1.(24 hours)
- Determine whether there is diurnal variability in muscle insulin signaling(24 hours)