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Alvocidib Biomarker-driven Phase 2 AML Study

Phase 2
Terminated
Conditions
Acute Myeloid Leukemia
Interventions
Registration Number
NCT02520011
Lead Sponsor
Sumitomo Pharma America, Inc.
Brief Summary

The purpose of this two-stage Phase 2 study is to assess the clinical response (Complete Remission) of ACM (Alvocidib/Cytarabine/Mitoxantrone) compared to CM (Cytarabine/Mitoxantrone) treatment in refractory or relapsed AML patients with demonstrated MCL-1 dependence of ≥ 30% by mitochondrial profiling in bone marrow.

Detailed Description

In Stage 1 of the study, all eligible AML patients with demonstrated MCL-1 dependence of ≥ 30% by mitochondrial profiling in bone marrow will receive treatment with ACM.

In Stage 2, all eligible AML patients with demonstrated MCL-1 dependence of ≥ 30% by mitochondrial profiling in bone marrow will be randomized 1:1 to receive either treatment with ACM or CM.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
104
Inclusion Criteria
  1. Be between the ages of ≥18 and ≤65 years

  2. Have an established, pathologically confirmed diagnoses of AML by World Health Organization (WHO) criteria excluding acute promyelocytic leukemia (APL-M3) with a bone marrow of >5% blasts based on histology or flow cytometry

  3. Be in first relapse (within 24 months of CR) or have failed induction therapy* (no CR or CRi after treatment with an intensive regimen (eg, anthracycline/cytarabine ± etoposide, gemtuzumab ozogamicin, or cladribine).

    *Induction therapy may involve 1 or 2 cycles of the same regimen. Efficacy assessment of induction therapy must be >21 days from the start of the previous induction cycle.

  4. Demonstrate MCL-1 dependence of ≥30% by mitochondrial profiling in bone marrow.

  5. Have an Eastern Cooperative Oncology Group (ECOG) performance status (PS) ≤2

  6. Have a serum creatinine level ≤1.8 mg/dL

  7. Have an alanine aminotransferase (ALT)/aspartate aminotransferase (AST) level ≤5 times upper limit of normal (ULN)

  8. Have a total bilirubin level ≤2.0 mg/dL (unless secondary to Gilbert syndrome, hemolysis, or leukemia)

  9. Have a left ventricular ejection fraction (LVEF) >45% by echocardiogram (ECHO) or multigated acquisition (MUGA) scan

  10. Be nonfertile or agree to use an adequate method of contraception. Sexually active patients and their partners must use an effective method of contraception associated with a low failure rate during and for at least 6 months after completion of study therapy.

  11. Be able to comply with the requirements of the entire study.

  12. Provide written informed consent prior to any study related procedure.

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Exclusion Criteria
  1. Received more than 2 cycles of induction therapy for AML. Investigational agents as part of front-line therapy for AML may by acceptable following discussion with the Medical Monitor. Hydroxyurea is permitted (see #5 below).
  2. Received any previous treatment with alvocidib or any other CDK inhibitor
  3. Received a hematopoietic stem cell transplant within the previous 2 months
  4. Have clinically significant graft versus host disease (GVHD), or GVHD requiring initiation or escalation of treatment within the last 21 days
  5. Require concomitant chemotherapy, radiation therapy, or immunotherapy. Hydroxyurea is allowed up to the evening before starting (but not within 12 hours) of starting treatment on either arm.
  6. Received >360 mg/m2 equivalents of daunorubicin
  7. Have a peripheral blast count of >30,000/mm3 (may use hydroxyurea as in #5 above)
  8. Received antileukemic therapy within the last 3 weeks (with the exception of hydroxyurea or if the patient has definite refractory disease). Refractory patients who received therapy within the last 3 weeks may be eligible with prior approval of the Medical Monitor.
  9. Diagnosed with acute promyelocytic leukemia (APL, M3)
  10. Have active central nervous system (CNS) leukemia
  11. Have evidence of uncontrolled disseminated intravascular coagulation
  12. Have an active, uncontrolled infection
  13. Have other life-threatening illness
  14. Have other active malignancies or diagnosed with other malignancies within the last 6 months, except nonmelanoma skin cancer or cervical intraepithelial neoplasia
  15. Have mental deficits and/or psychiatric history that may compromise the ability to give written informed consent or to comply with the study protocol.
  16. Are pregnant and/or nursing
  17. Have received any live vaccine within 14 days prior to first study drug administration.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
CM (Stage 2)MitoxantroneC: cytarabine (ara-c), 2 gm/m2 by continuous IV infusion over 72 hours on Days 1-3; M: mitoxantrone (mitoxantrone hydrochloride), 40 mg/m2 by IV infusion over 1-2 hours starting 12 hours after completing cytarabine
CM (Stage 2)CytarabineC: cytarabine (ara-c), 2 gm/m2 by continuous IV infusion over 72 hours on Days 1-3; M: mitoxantrone (mitoxantrone hydrochloride), 40 mg/m2 by IV infusion over 1-2 hours starting 12 hours after completing cytarabine
ACM (Stage 1 / Stage 2)CytarabineA: alvocidib, 30 mg/m2 as a 30 minute intravenous (IV) bolus followed by 60 mg/m2 over 4 hours as an IV infusion administered daily on Days 1-3; C: cytarabine (ara-c), 2 gm/m2 by continuous IV infusion over 72 hours on Days 6-8; M: mitoxantrone (mitoxantrone hydrochloride), 40 mg/m2 by IV infusion over 1-2 hours starting 12 hours after completing cytarabine
ACM (Stage 1 / Stage 2)MitoxantroneA: alvocidib, 30 mg/m2 as a 30 minute intravenous (IV) bolus followed by 60 mg/m2 over 4 hours as an IV infusion administered daily on Days 1-3; C: cytarabine (ara-c), 2 gm/m2 by continuous IV infusion over 72 hours on Days 6-8; M: mitoxantrone (mitoxantrone hydrochloride), 40 mg/m2 by IV infusion over 1-2 hours starting 12 hours after completing cytarabine
ACM (Stage 1 / Stage 2)AlvocidibA: alvocidib, 30 mg/m2 as a 30 minute intravenous (IV) bolus followed by 60 mg/m2 over 4 hours as an IV infusion administered daily on Days 1-3; C: cytarabine (ara-c), 2 gm/m2 by continuous IV infusion over 72 hours on Days 6-8; M: mitoxantrone (mitoxantrone hydrochloride), 40 mg/m2 by IV infusion over 1-2 hours starting 12 hours after completing cytarabine
Primary Outcome Measures
NameTimeMethod
Complete Response (CR) Rate in Patients With Relapsed or Refractory AMLBest response after at least 1 cycle through study completion approximately 4 years

Complete Remission (CR) rate = Percentage of patients achieving CR after Cycle 1 as defined in Stage 1 by the International Working Group (IWG) Criteria and 2010 European LeukemiaNet (EN) criteria in patients with relapsed or refractory AML with MCL-1 dependence \>30% and in Stage 2 by the 2017 ELN criteria.

The study was terminated in January 2020 due to a steady and marked reduction in enrollment. Thus, the efficacy endpoints could not be analyzed. As sufficient efficacy results were not available to analyze patients based on the percentage of MCL-1 dependency the treatment efficacy was summarized by distributing the safety population into 6 groups based on whether the patients received the ACM vs CM regimen and their disease stages at study entry.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (38)

Honor Health Research Institute

🇺🇸

Scottsdale, Arizona, United States

Mayo Clinic Florida

🇺🇸

Jacksonville, Florida, United States

Morristown Cancer Center

🇺🇸

Morristown, New Jersey, United States

Hospital Regional Universitario de Malaga

🇪🇸

Málaga, Malaga, Spain

Institut Catala d'Oncologia

🇪🇸

Badalona, Spain

Hospital Clinic de Barcelona

🇪🇸

Barcelona, Spain

Hospital Universitario Central de Asturias - HUCA

🇪🇸

Oviedo, Spain

Hospital Clinico Universitario de Salamanca

🇪🇸

Salamanca, Spain

Hospital Universitari i Politècnic La Fe

🇪🇸

Valencia, Spain

University Hospitals of Wales

🇬🇧

Cardiff, Wales, United Kingdom

Univ Hospital of Bristol

🇬🇧

Bristol, United Kingdom

Guys Hospital St. Thomas

🇬🇧

London, United Kingdom

Northwestern Memorial Hospital

🇺🇸

Chicago, Illinois, United States

Sidney Kimmel Cancer Center at Johns Hopkins

🇺🇸

Baltimore, Maryland, United States

East Carolina University

🇺🇸

Greenville, North Carolina, United States

Cleveland Clinic Foundation

🇺🇸

Cleveland, Ohio, United States

Ohio State University

🇺🇸

Columbus, Ohio, United States

Baylor Sammons Cancer Center

🇺🇸

Dallas, Texas, United States

Princess Margaret Cancer Center

🇨🇦

Toronto, Ontario, Canada

MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

University of California Los Angeles (UCLA)

🇺🇸

Los Angeles, California, United States

Northside Hospital

🇺🇸

Atlanta, Georgia, United States

University of Iowa

🇺🇸

Iowa City, Iowa, United States

Roswell Park Cancer Center Institute

🇺🇸

Buffalo, New York, United States

Columbia University Medical Center

🇺🇸

New York, New York, United States

West Penn Allegheny Hospital

🇺🇸

Pittsburgh, Pennsylvania, United States

University of California San Diego UCSD

🇺🇸

San Diego, California, United States

Mayo Clinic Rochester

🇺🇸

Rochester, Minnesota, United States

University of Nebraska Medical Center

🇺🇸

Omaha, Nebraska, United States

Duke

🇺🇸

Durham, North Carolina, United States

Medical College of Wisconsin

🇺🇸

Milwaukee, Wisconsin, United States

Hudson Valley Cancer Center

🇺🇸

Hawthorne, New York, United States

Complejo Hospitalario Universitario de Albacete

🇪🇸

Albacete, Spain

Hospital San Pedro de Alcantara

🇪🇸

Cáceres, Spain

University of Michigan

🇺🇸

Ann Arbor, Michigan, United States

Ochsner Clinic Foundation

🇺🇸

New Orleans, Louisiana, United States

University of North Carolina

🇺🇸

Chapel Hill, North Carolina, United States

University of Kansas Medical Center

🇺🇸

Westwood, Kansas, United States

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