The EFFECTS of GAMBIR CATECHIN (UNCARIA GAMBIR ROXB) SUPPLEMENTATION on COGNITIVE FUNCTION, EXPRESSION of APP, MAPT, BDNF, IL-10, TGF-Β GENES, and PLASMA MDA LEVELS in the ELDERLY.
- Conditions
- Elderly Individuals Aged 60 Years or OlderMild Cognitive Impairment (MCI)
- Interventions
- Dietary Supplement: catechin extracts (health food)Other: plasebo
- Registration Number
- NCT06604390
- Lead Sponsor
- Rahmi Novita Yusuf
- Brief Summary
Objective The primary objective of this clinical trial is to investigate the effects of gambir catechin administration on cognitive function, the expression of amyloid precursor protein (APP), microtubule-associated protein tau (MAPT), brain-derived neurotrophic factor (BDNF), interleukin-10 (IL-10), transforming growth factor beta (TGF-beta), and plasma malondialdehyde (MDA) levels in elderly individuals.
Research Questions
The primary research questions to be addressed in this study are as follows:
1. Does the administration of gambir catechin (Uncaria gambir Roxb) improve cognitive function as measured by the Montreal Cognitive Assessment-Indonesia (MoCA-INA) in the elderly after 12 weeks of intervention?
2. Does the administration of gambir catechin (Uncaria gambir Roxb) affect the expression of APP, MAPT, BDNF, IL-10, and TGF-beta genes in the elderly after 12 weeks of intervention?
3. Does the administration of gambir catechin (Uncaria gambir Roxb) influence plasma MDA levels in the elderly after 12 weeks of intervention? Methodology
To answer these questions, researchers will compare the effects of gambir catechin with a placebo (an inert substance). This double-blind, randomized controlled trial will involve:
* Participant Recruitment: Elderly individuals will be recruited and randomly assigned to either the gambir catechin or placebo group.
* Intervention: Participants will receive daily doses of gambir catechin or a placebo for 12 weeks.
* Assessments: Cognitive function, gene expression, and plasma MDA levels will be assessed at baseline and after 12 weeks of intervention.
* Data Analysis: The collected data will be analyzed using appropriate statistical methods to determine the effects of gambir catechin.
Participant Involvement
Participants will be required to:
* Consume the assigned supplement (gambir catechin or placebo) daily.
* Attend regular follow-up visits at the research center.
* Report any adverse events or changes in their health during the study period.
- Detailed Description
Effects of Gambier Catechin (Uncaria gambir Roxb) Supplementation on Cognitive Function, APP, MAPT, BDNF, IL-10, TGF-β Gene Expression, and Plasma MDA Levels in the Elderly
1. Background Problem: The increasing prevalence of mild cognitive impairment among the elderly and its impact on their quality of life.
Research Gap: A lack of research on the utilization of local plants, specifically catechin, an active compound found in gambier leaves, which possesses antioxidant and anti-inflammatory properties and may enhance cognitive function in the elderly population in Indonesia.
Research Objective: To investigate the effects of gambier catechin (Uncaria gambir Roxb) supplementation on cognitive function, APP, MAPT, BDNF, IL-10, TGF-β gene expression, and plasma MDA levels in the elderly.
2. Hypothesis
1. There is an effect of gambier catechin (Uncaria gambir Roxb) supplementation on improving cognitive function as measured by the MoCA-INA test in the elderly after 12 weeks of supplementation.
2. There is an effect of gambier catechin (Uncaria gambir Roxb) supplementation on decreasing the expression of APP, MAPT, TGF-β genes and increasing the expression of BDNF, IL-10 genes in the elderly after 12 weeks of supplementation.
3. There is an effect of gambier catechin (Uncaria gambir Roxb) supplementation on decreasing plasma MDA levels in the elderly after 12 weeks of supplementation.
3. Research Method Research Design: A randomized controlled trial (RCT) design will be used. Population and Sample:The target population for this study is the elderly aged 60 years and above with mild cognitive impairment (MoCA-INA score of 20-25) residing in Padang City, West Sumatra, Indonesia.
4. Research Procedure
1. Recruitment and Screening
1. Participants will be recruited from community centers, senior citizen clubs, and public health facilities in Padang City.
2. Eligibility criteria will include:
1. Age 60 years and above
2. Mild cognitive impairment (MoCA-INA score of 20-25)
3. No history of neurological or psychiatric disorders
4. No history of substance abuse or chronic medical conditions that may interfere with cognitive function
5. Willingness to participate in the study and provide informed consent
2. Baseline Assessments
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1. Demographic information will be collected.
2. Cognitive function will be assessed using the Montreal Cognitive Assessment-Indonesia (MoCA-INA) test.
3. Blood samples will be drawn for biochemical analysis, including plasma MDA levels.
4. Genetic analysis will be conducted to assess the expression of APP, MAPT, BDNF, IL-10, and TGF-β genes.
c. Intervention
1. Participants will be randomly assigned to either the intervention or control group.
2. The intervention group will receive gambier catechin supplementation (Uncaria gambir Roxb) at a daily dose of \[Insert dosage\] for 12 weeks.
3. The control group will receive a placebo. d. Follow-up Assessments
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1. Cognitive function will be reassessed using the MoCA-INA test at 12 weeks.
2. Blood samples will be drawn for biochemical analysis and genetic analysis as described in the baseline assessments.
5. Data Analysis
1. Descriptive statistics will be used to summarize demographic data, cognitive function scores, and biochemical parameters.
2. Inferential statistics, including t-tests and ANOVA, will be used to compare the differences between the intervention and control groups.
3. Correlation analysis will be conducted to examine the relationship between cognitive function, genetic expression, and plasma MDA levels.
6. Ethical Considerations
1. The study will be conducted in accordance with ethical principles and guidelines, including the Declaration of Helsinki.
2. Informed consent will be obtained from all participants.
3. Participant confidentiality will be maintained throughout the study.
4. Any adverse events will be monitored and reported.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 64
- Age 60 years and above
- Mild cognitive impairment (MoCA-INA score of 20-25)
- No history of neurological or psychiatric disorders
- No history of substance abuse or chronic medical conditions that may interfere with cognitive function
- Willingness to participate in the study and provide informed consent
- Participants who did not take Catechin Capsules for two days in a row
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Arm 1: Catechin Group Arm 2: Placebo Group catechin extracts (health food) Arm 1: Catechin Group. Participants in this group received one capsule of gambir catechin daily, with a dosage of 224 mg. Arm 2: Placebo Group. Participants in this group received one placebo capsule daily, with a dosage of 224 mg. Arm 1: Catechin Group Arm 2: Placebo Group plasebo Arm 1: Catechin Group. Participants in this group received one capsule of gambir catechin daily, with a dosage of 224 mg. Arm 2: Placebo Group. Participants in this group received one placebo capsule daily, with a dosage of 224 mg. Effects of Gambier Catechin Supplementation on Cognitive Function, APP, MAPT, catechin extracts (health food) Gambir purified with a minimum of 90% (+)-Catechin content. Dosage of 224 mg catechin per capsule, once daily. Effects of Gambier Catechin Supplementation on Cognitive Function, APP, MAPT, plasebo Gambir purified with a minimum of 90% (+)-Catechin content. Dosage of 224 mg catechin per capsule, once daily.
- Primary Outcome Measures
Name Time Method To determine the effect of gambir catechin (Uncaria gambir Roxb) supplementation on cognitive function using the MoCA-INA test in the elderly after 12 weeks of supplementation April to september 2024 ( 6 month) scor MoCA: 20 to 25
To determine the effect of gambir catechin (Uncaria gambir Roxb) supplementation on the expression of APP, MAPT, BDNF, IL-10, and TGF-β genes in the elderly after 12 weeks of supplementation. April to september 2024 ( 6 month) Gene expression was measured using PCR
To determine the effect of gambir catechin (Uncaria gambir Roxb) supplementation on plasma MDA levels in the elderly after 12 weeks of supplementation. April to september 2024 ( 6 month) Plasma MDA levels, as a marker of lipid peroxidation, were measured using a UV-vis spectrophotometer and the TBARS assay
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Andalas Univercity
🇮🇩Padang, West Sumatera, Indonesia