The Effects of Animal- and Microbial-based Protein Sources on Whole-body Protein Metabolism in Healthy Adults
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Sponsor
- Daniel Moore
- Enrollment
- 13
- Locations
- 1
- Primary Endpoint
- 1. Phenylalanine excretion (umol/kg/h)
Study Overview
Brief Summary
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- Single (Participant)
Eligibility Criteria
- Ages
- 18 Years to 45 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Healthy, male and female, recreationally-active participants.
- •Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
- •Participants will be aged 18-45 years old.
- •Participants are willing to abide by the compliance rules of this study.
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
- •Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).
Exclusion Criteria
- •Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
- •Self-reported regular tobacco use.
- •Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
- •Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- •Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
- •Use of COCs containing >21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
- •Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
- •Not currently or previously (in the past 6 months) on a vegan diet
Arms & Interventions
Yeast Protein Supplement
Participants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.
Intervention: Yeast Protein Supplement (Dietary Supplement)
Whey Protein Isolate Supplement
Participants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours.
Intervention: Whey Protein Isolate Supplement (Dietary Supplement)
Collagen Hydrolysate Supplement
Participants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.
Intervention: Collagen Hydrolysate Supplement (Dietary Supplement)
Outcomes
Primary Outcomes
1. Phenylalanine excretion (umol/kg/h)
Time Frame: 8 hours
Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Phenylalanine oxidation (umol/kg/h)
Time Frame: 8 hours
Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.
Secondary Outcomes
No secondary outcomes reported
Investigators
Daniel Moore
Principal Investigator
University of Toronto