ARE SMAD2, SMAD3 AND TGF-β GENE EXPRESSION VALUABLE IN THE MANAGEMENT OF THE URGE URINARY INCONTINENCE?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- SMAD3
研究概览
简要总结
Urge urinary incontinence (UUI) is a common health problem. Changes in collagen metabolism in pelvic support organs, such as uterosacral ligaments (USLs), might be responsible for the complex pathophysiology of UUI. The TGF-β pathway is involved in collagen synthesis and degradation. The Transforming Growth Family- β (TGF-β) superfamily has essential intracellular signaling components, such as newly identified SMAD family members. We evaluated the changes in the levels of TGF-β and SMAD gene and protein expression in the USL of patients with concomitant pelvic organ prolapse (POP) and UUI.
详细描述
This study included 10 patients who had been diagnosed with POP and UUI in the study group and 14 asymptomatic women without complaints of POP and UUI in the control group. Biopsy samples were collected from bilateral USL tissues during vaginal or abdominal hysterectomy. Total RNA was extracted from USL tissue and analyzed by qPCR. The protein expression levels were also analyzed with ELISA.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •patients who had been diagnosed with POP and UUI
- •asymptomatic women without complaints of POP and UUI
排除标准
- •Women who have SUI,
- •a history of gynecologic malignancy,
- •endometriosis,
- •connective tissue disorders,
- •previous pelvic inflammatory disease,
- •pelvic radiation anamnesis,
- •previous surgery for POP or UI,
- •those on steroid therapy and hormone replacement therapy
结局指标
主要结局
SMAD3
时间窗: intraoperative
tissue expression and protein level
TGF-ß1 mRNA Expression Levels
时间窗: intraoperative
tissue expression and protein level
SMAD2
时间窗: intraoperative
tissue expression and protein level
次要结局
未报告次要终点
研究者
Melike Nur AKIN
Dr Melike Nur Akın
Muğla Sıtkı Koçman University