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Characterization of the Microbiome in Peritoneum, Jejunum, Rectum and Stool

Active, not recruiting
Conditions
Chronic Pancreatitis
Obesity
Fatty Liver, Nonalcoholic
Diabetes Mellitus
Leaky Gut Syndrome
Interventions
Diagnostic Test: Microbial Swab
Registration Number
NCT04067440
Lead Sponsor
University Hospital, Saarland
Brief Summary

Characterization of the human microbiome in the jejunum and comparison to the microbiome in the rectum and stool in order to see how the microbial communities change within the intestines.

Detailed Description

Subjects to undergo surgery with opening of the jejunum will be sampled with sterile swabs from peritoneum and the inside of the jejunum. A swab will also be taken from the rectum.

The peritoneum is regarded as a sterile place until today. We try to either verify this, we also could find a scientific base for theories like leaky gut or other forms of bacterial migration through the intestinal wall.

Many of these patients undergo bariatric surgery. Many of them do have a dramatic change in glucose metabolism immediate after surgery. Therefore stool swabs will be taken before and 5 days after surgery.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
30
Inclusion Criteria
  • abdominal surgery containing opening of jejunum
  • singing of consent
Exclusion Criteria
  • inflammative condition (appendicitis, pancreatitis, Crohn, Colitis ulcerous, cholecystitis and similar)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
PatientsMicrobial SwabSubjects to undergo surgery including opening of the jejunum with non-inflammative condition.
Primary Outcome Measures
NameTimeMethod
Characterization of microbiomeintra-operative

Previously uncharacterized sites: jejunum, peritoneum

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Department of General, Visceral, Vascular and Pediatric Surgery, University of Saarland,

🇩🇪

Homburg/Saar, Germany

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