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Regulation of Fat-stimulated Neurotensin Secretion in Healthy Subjects

Not Applicable
Completed
Conditions
Healthy
Registration Number
NCT00541762
Lead Sponsor
University Hospital, Basel, Switzerland
Brief Summary

Context: Cholecystokinin (CCK) and neurotensin are stimulated during meal intake by the presence of fat in the small intestine. The sequence of events suggests that fat hydrolysis is crucial for triggering the release.

Objectives: The aim of this study was therefore to investigate whether CCK mediated the effect of intraduodenal (ID) fat on neurotensin secretion via CCK-1 receptors.

Detailed Description

Setting: Single center study; 34 male volunteers were studied in consecutive, randomized, double blind, crossover studies.

Subjects and Methods: CCK and neurotensin release were quantified in: 1) 12 subjects receiving an ID fat infusion with or without 60 mg orlistat, an irreversible inhibitor of gastrointestinal lipases, in comparison to vehicle. 2) 12 subjects receiving ID long chain fatty acids (LCF), ID medium chain fatty acids (MCF) or ID vehicle. 3) 10 subjects receiving ID LCF with and without the CCK-1 receptor antagonist dexloxiglumide (DEXLOX) or ID vehicle plus IV saline (placebo). Hormone concentrations were measured by specific RIA systems.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
34
Inclusion Criteria
  • Healthy male subjects
Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Neurotensin plasma concentrationsChange in plasma cocnentrations over 2-3 hours
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Clinical Research Center, University Hospital

🇨🇭

Basel, Switzerland

Clinical Research Center, University Hospital
🇨🇭Basel, Switzerland

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