Regulation of Fat-stimulated Neurotensin Secretion in Healthy Subjects
- Conditions
- Healthy
- Interventions
- Dietary Supplement: Fat perfusion to the small intestine
- Registration Number
- NCT00541762
- Lead Sponsor
- University Hospital, Basel, Switzerland
- Brief Summary
Context: Cholecystokinin (CCK) and neurotensin are stimulated during meal intake by the presence of fat in the small intestine. The sequence of events suggests that fat hydrolysis is crucial for triggering the release.
Objectives: The aim of this study was therefore to investigate whether CCK mediated the effect of intraduodenal (ID) fat on neurotensin secretion via CCK-1 receptors.
- Detailed Description
Setting: Single center study; 34 male volunteers were studied in consecutive, randomized, double blind, crossover studies.
Subjects and Methods: CCK and neurotensin release were quantified in: 1) 12 subjects receiving an ID fat infusion with or without 60 mg orlistat, an irreversible inhibitor of gastrointestinal lipases, in comparison to vehicle. 2) 12 subjects receiving ID long chain fatty acids (LCF), ID medium chain fatty acids (MCF) or ID vehicle. 3) 10 subjects receiving ID LCF with and without the CCK-1 receptor antagonist dexloxiglumide (DEXLOX) or ID vehicle plus IV saline (placebo). Hormone concentrations were measured by specific RIA systems.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 34
- Healthy male subjects
Not provided
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description A, 3; B, 3; C, 3 Fat perfusion to the small intestine A, 3: Fat with and without orlistat or placebo. B, 3: LCF vs MCF vs placebo. C, 3: LCF with and without DEXLOX or placebo.
- Primary Outcome Measures
Name Time Method Neurotensin plasma concentrations Change in plasma cocnentrations over 2-3 hours
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Clinical Research Center, University Hospital
🇨🇭Basel, Switzerland