NCT00611117已完成不适用
Mechanisms of the Effect of Physical Activity on the Adaptation to a High-Fat Diet
适应症
干预措施
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 17
- 试验地点
- 1
- 主要终点
- To determine the effects of a high fat diet [at both low and high physical activity] on • Sk muscle PGC1α and PDK4 mRNA and protein • Glucose oxidation • Fatty acid oxidation • Hepatic and skeletal muscle lipid • VO2 max • Mitochondr
研究概览
简要总结
The study is designed to determine the effect of high physical activity level vs. low physical activity level on the adaptation to a high fat diet.
详细描述
A high fat diet is linked to weight gain and obesity. An adjustment to the acute exposure to high fat diet is not abrupt and takes time. In a previous study from our laboratory, it has been shown that high level of physical activity can accelerate the adaptation to a high fat diet by increasing fat oxidation. In this study we will determine the mechanism involved in this adjustment. Our hypotheses are:
- High fat diets decrease skeletal muscle glucose metabolism and decrease mitochondrial biogenesis through the upregulation of PDK4 and downregulation of PGC1α; increased physical activity will prevent these deleterious effects.
- A high fat diet will increase hepatic and skeletal muscle lipid; increased physical activity will prevent these deleterious effects.
- These effects are more pronounced in individuals with a low mitochondrial content
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Care Provider)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Both genders and all races will be invited to participate
- •BMI > 19 and < 35 kg/m2
- •Age 18-35
- •Women will be asked to participate in the follicular phase of the menstrual cycle as determined by menstrual history and a negative pregnancy test will be recorded prior to participation
- •Willing and able to walk on a treadmill at a pace of 3 mph at 3%grade for 3-4 hours/day for 3 consecutive days
- •Willing to eat only and all foods provided by Pennington for 9 days on 2 occasions (18 days)
- •Willing to stop alcohol consumption for the duration of the study
- •Able come to Pennington for meals and tests for 20 days 12 of these inpatient.
排除标准
- •Significant renal, hepatic, endocrine, pulmonary, cardiac or hematological disease (for the consent form you will have to use kidney, liver, etc )
- •You abuse alcohol or illegal drugs or are unable to avoid alcohol or caffeine prior to testing
- •Unable or unlikely to eat all and only food provided by the PBRC metabolic kitchen.
- •Pregnant or planning to become pregnant
- •Corticosteroid use in previous two months
- •Chronic use of anti-diabetic, anti-hypertensive, or other medications known to affect fat metabolism
- •Use of Depo-Provera, hormone implants or estrogen replacement therapy
- •Irregular menstrual cycles
- •Post-menopausal women
- •Weight gain or loss of > 3kg in the last 6 months
- •Unable or unwilling to perform treadmill walking at the required speed and incline to achieve 1.8*RMR
- •History or family history of blood clots deep vein thrombosis or pulmonary embolism)
- •Poor circulation, bypass operation on legs, blood coagulation disorders, diagnosed peripheral arterial or vascular disease, cramping pain in your muscle during exercise or nerve damage in your legs.
- •You had major surgery on the abdomen, pelvis or legs within previous 3 months.
- •You have cancer
- •You have rheumatoid disease/ walking or joint problems
- •You are claustrophobic
- •You are depressed or have an eating disorder
- •You demonstrate a style of interpersonal relationships that would inhibit successful completion of the study
- •For the MRS / MRI, the following exclusion criteria apply:
- •Individuals who have a heart pacemaker, defibrillator, or non-removable hearing aid
- •Individuals with any clips or metal plates in their head
- •Individuals who have any artificial limbs or prosthetic devices
- •Individuals who were ever injured by a metallic foreign body which was not removed
- •Individuals with bridgework held in place by magnets
研究组 & 干预措施
1
Experimental
High intensity exercise and high fat diet
干预措施: Physical Activity Level (Other)
2
Experimental
Low intensity exercise and high fat diet
干预措施: Physical Activity Level (Other)
结局指标
主要结局
To determine the effects of a high fat diet [at both low and high physical activity] on • Sk muscle PGC1α and PDK4 mRNA and protein • Glucose oxidation • Fatty acid oxidation • Hepatic and skeletal muscle lipid • VO2 max • Mitochondr
时间窗: One Year
次要结局
未报告次要终点
研究者
研究点 (1)
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