A New Micro Swim-up Procedure for Sperm Preparation in ICSI Treatment
- Conditions
- Microswimup Procedure Efficacy
- Registration Number
- NCT02672124
- Lead Sponsor
- Cervesi Hospital, Cattolica, Italy
- Brief Summary
The aim of this study is to evaluate the microbiological safety and the efficacy of a new not expensive, easy and fast technique (Direct Microswimup) for sperm preparation in In Vitro Fertilization (IVF) treatments without the use of centrifuge, able to simplify the procedure and to avoid mismatches by ensuring the same results of the standard techniques in terms of fertilization rate, blastulation rate, pregnancy/abortion rate, cryostorage rate, and a better euploid status of the embryos.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 100
- at least 8 oocytes retrieved at pickup
- oocyte insemination with ICSI technique
- only elective single embryo transfer (eSET) at blastocyst stage
- sperm concentration ≥ 1 X 106 /ml
- absence of antibiotic treatment in the previous four weeks for male patients
- recurrent miscarriages
- more than three in vitro fertilization treatment
- azoospermia
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method microbiological test 3 months A standard bacteriological culture \[based on inoculation of blood and "chocolate" agar plates and Brain-Heart Infusion broth, followed by incubation at 37°C for up to 72 hours\] was performed in order to investigate the presence and amount of colonizing bacteria delivered with the semen at ejaculation and after treatment
- Secondary Outcome Measures
Name Time Method semen treatment efficacy 6 months number of obtain blastocysts (%) and number of pregnancy (%)
Trial Locations
- Locations (2)
Cervesi Hospital IVF Unit
🇮🇹Cattolica, Rimini, Italy
Primiterra Mariangela
🇮🇹Cattolica, Rimini, Italy