Comparison of Nylon Flocked Swabs and Saline Aspirates for Detection Respiratory Viruses

Not Applicable

• Conditions: Respiratory Syncytial Virus; Human Metapneumovirus; Influenza; Bronchiolitis; Pediatric
• Interventions: Device: Nylon Flocked swab (Nasal secretion sampling)

• Registration Number: NCT00613184
• Lead Sponsor: Kern Medical Center

Brief Summary

Collection of nasal secretions from infants and toddlers for viral testing is usually done using the nasal washing technique described by Hall in 1975. This is cumbersome. Previous attempts to use swabs have been unsuccessful because the swabs didn't work well. A newly designed swab may work better and in this study we compare the new swab with the old style nasal washing.

.

Detailed Description

Collection of nasal secretions from infants and toddlers for viral testing is typically performed using the nasal saline aspirate technique described by Hall in 1975.

Nylon flocked swabs (NFS) and universal transport medium for room temperature (UTM-RT) (Copan Medical, Murrieta, CA) storage media have been found to be an effective collection and transport method for bacteria causing sexually transmitted infections.

We adapted these swabs and storage medium to collect respiratory viruses from children less than18 months old and compared detection rates using NFS and traditional nasal aspirates. We will determine the relative roles of the UTM-RT and NFS release and therefore measured viral detection rates of common respiratory pathogens in traditional saline aspirates stored in UTM-RT.

Our primary hypothesis is that nasal secretions collection using NFS stored in UTM-RT will lead to a higher detection rate of the respiratory viruses we arestudying; namely RSV, Influenza and human metapneumovirus from than collection of unpreserved saline nasal aspirates in children less than 18 months of age.

Study Timeline

• Study Start: 2006-11
• Status Verified: 2008-01
• Primary Completion: 2008-01
• Study Completion: 2008-01
• Study First Submitted: 2008-01-29
• Study First Submitted QC: 2008-02-11
• Last Update Submitted: 2008-02-11
• Study First Posted: 2008-02-12
• Last Update Posted: 2008-02-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 150

Inclusion Criteria

• Treating clinican ordered RSV antigen testing

Exclusion Criteria

• Refusal of consent
• Age > 18 months

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
4	Nylon Flocked swab (Nasal secretion sampling)	Nasal Wash R Nylon flocked swab L
1	Nylon Flocked swab (Nasal secretion sampling)	Nylon Flocked swab Left Nasal Wash right
2	Nylon Flocked swab (Nasal secretion sampling)	Nylon Flocked swab R Nasal Wash L
3	Nylon Flocked swab (Nasal secretion sampling)	Nasal Wash Left Nylon Flocked swab Right

Primary Outcome Measures

Name	Time	Method
Viral detection rate by PCR	0 not applicable