The role of innate immunity in the pathogenesis of acute respiratory syncytial virus lower respiratory tract infection: Repeated measurements of nasopharyngeal type 1 interferon levels in RSV infections

Completed

• Conditions: Innate immune response; Viral respiratory tract infection; 10024970

• Registration Number: NL-OMON35329
• Lead Sponsor: RSV Research Group van het Wilhelmina Kinderziekenhuis Utrecht, UMCU, onder leiding van Prof. dr. J.L.L. Kimpen, promotor

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 24 June 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 400

Inclusion Criteria

Child = under the age of 13 months (exception: <5 years old for group 3)
Adult = over the age of 18 years
Upper respiratory infection = symptoms of rhinitis with or without mild cough
Lower respiratory infecton = symptoms of hypoxemia, tachypnea, dyspnea, severe cough, and/or repeated apneu's, fever

Exclusion Criteria

Child = previous airway morbidity or severe co-morbidity (exception: no exclusion criteria for group 3)
Adult = None

Study & Design

• Study Type: Observational non invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. Disease severity:<br /><br>Children: Standardised Respiratory Distress Assessment Instrument (RDAI) Score<br /><br>Elderly: Standardised Adult Physiology And Chronic Health (APACHE) Score,<br /><br>Respiratory Parameter<br /><br><br /><br>2. Virological analysis (nasopharyngeal material):<br /><br>Quantitative RT-PCR on RSV, hMPV, rhinovirus, adenovirus, PIV 1-4, Flu A en B,<br /><br>en coronavirus<br /><br><br /><br>3. Immunological analysis/cytokine concentrations (nasopharyngeal material):<br /><br>Interferon-alfa using commercial ELISA<br /><br>IL-1a&beta;/a, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12*70, IL-13, IL-15, IL-17,<br /><br>IL-18, IFN-&gamma; and TNF-a using Luminex<br /><br><br /><br>4. Immunological analysis (blood):<br /><br>Leucocytes and differentiation<br /><br>Immunophenotyping<br /><br>Toll-like receptor responses</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>na</p><br>