Peanut Protein Supplementation to Augment Muscle Growth and Improve Markers of Muscle Quality and Health in Older Adults
- Conditions
- AgingSarcopenia
- Interventions
- Dietary Supplement: Peanut Protein PowderBehavioral: Full body resistance training
- Registration Number
- NCT04015479
- Lead Sponsor
- Auburn University
- Brief Summary
This study will evaluate the adaptations in skeletal muscle that occur in response to 10 weeks of weight training with or without peanut protein supplementation in older adult men and women.
- Detailed Description
Aging is associated with declines in muscle mass, physical strength and physical function. Adequate quality protein intake in aging adults is critical to preventing functional decline. Peanuts provide a unique blend of amino acids that can provide several health benefits to aging adults. While supplementing with peanut protein (PP) powder as part of a resistance training program may increase myofibrillar protein synthesis (i.e., the gold standard molecular assessment in deciphering a muscle-building response), and improve skeletal muscle quality and body composition, no study to date has made this determination.
This is a two-phase study using both novel and conventional methods to assess how PP supplementation affects muscle tissue in older individuals who engage in resistance training. These two phases will be conducted as part of a 10-week randomized controlled trial in which men and women aged 50 years and older (n=60), will be stratified by gender and randomized to a resistance training intervention (whole body, two days per week) with PP powder (72g daily; n=15 males, n=15 females) provided during the intervention (immediate group, IG) or after the intervention (wait-list control, WLC, n=15 males, n=15 females). The aims of this study are to determine the acute (deuterium oxide tracer) and chronic (peripheral quantitative computed tomography) effects of PP during resistance training on skeletal muscle myofibrillar protein synthesis rates, changes in skeletal muscle size and quality, changes in whole and appendicular body composition (dual energy x-ray absorptiometry), changes in inflammatory markers and the fecal microbiome.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 41
- body mass index (body mass/height squared) less than 35 kg/m2
- resting blood pressure averaging less than 140/90 mmHg (with or without medication)
- known peanut allergy
- actively participating in resistance training for more than 2 days/week
- any known overt cardiovascular or metabolic disease
- metal implants that will interfere with x-ray procedures
- medically necessary radiation exposure in the last six months (except dental x-ray)
- any medical condition that would contradict participating in an exercise program, giving blood or donating a skeletal muscle biopsy (i.e. blood clotting disorder or taking blood thinners)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Immediate Intervention Group Peanut Protein Powder Participants will complete 10 weeks of twice-weekly whole body resistance training. Peanut protein powder (72g/day) will be provided for daily consumption during the study period Immediate Intervention Group Full body resistance training Participants will complete 10 weeks of twice-weekly whole body resistance training. Peanut protein powder (72g/day) will be provided for daily consumption during the study period Wait-list Control Group Full body resistance training Participants will complete 10 weeks of twice-weekly whole body resistance training. Peanut protein powder will be provided after the study period
- Primary Outcome Measures
Name Time Method Change in acute myofibrillar protein synthesis rates 24 hours Change in right leg vastus lateralis myofibrillar protein synthesis rates using the integrated deuterium oxide technique from biopsies immediately before and 24 hours after resistance exercise
Change in mid-thigh skeletal muscle area and quality 0-10 weeks peripheral quantitative computed tomography (pQCT) cross-sectional image of mid-right thigh assessed for total muscle cross-sectional area, subcutaneous adipose tissue area, total intra- and inter-muscular adipose tissue area and overall muscle density
- Secondary Outcome Measures
Name Time Method Change in fecal microbiome composition 0-10 weeks alpha- and beta-diversity of 16S bacterial rDNA
Change in whole-body and appendicular body composition 0-10 weeks dual energy x-ray absorptiometry (DXA)
Change in Type I and II Muscle Fiber Cross-Sectional Area 0-10 weeks Muscle biopsy immunofluorescent staining for determination of type I and type II muscle fiber cross sectional area (fCSA) as a cellular determinant of skeletal muscle hypertrophy
Change in leg extensor isokinetic dynamometry 0-10 weeks maximal isokinetic right leg extensions on an isokinetic dynamometer (BioDex)
Change in inflammatory biomarkers 0-10 weeks serum C-reactive protein, interleukin-6, tumor necrosis factor-alpha, plasma 8-hydroxy-2'deoxyguanosine
Trial Locations
- Locations (1)
Auburn University
🇺🇸Auburn, Alabama, United States