Re-evaluation of the Muscle-full Effect During Continuously Elevated Amino Acid Availability in Healthy Young Males
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 12
- 试验地点
- 1
- 主要终点
- Muscle protein synthesis during continuous elevated plasma amino acid avaialbility in healthy young males
研究概览
简要总结
Muscle tissue consists of proteins. These proteins are built up of a collection of smaller building blocks: amino acids. When protein is consumed, it gets digested and absorbed into the blood. The body can use these amino acids, by taking them up from thecirculation. By consuming sufficient protein through our diet, we ensure that the body is provided with enough amino acids to enable muscle protein building. Sufficient muscle protein synthesis is important for maintaining muscle function and strength.
Previous research has shown that when 20 to 25g of protein is eaten, muscle protein synthesis is maximized. It is therefore recommended to eat 20g of protein per meal. However, it is currently unclear what happens to muscle protein synthesis rates if multiple meals are eaten. When multiple meals are consumed, amino acids appear in the circulation for prolonged period of time.
Theoretically, when there are a high amino acid concentrations in the blood, muscle protein synthesis rates will increase. Contrary to this theory, a study more than 20 years ago showed otherwise. It was observed that muscle protein synthesis rates are only elevated for2 hours afterwhich they decrease again. This phenomenon was referred to as the "muscle-full" effect. Because this phenomenon is in contrast with more previous studies, the objective is to replicate that study. This is important so that nutritional advice for healthy, but also clinical populations in the future can be improved.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Aged between 18 - 35 years
- •Healthy (assessed based on routine medical questionnaire)
- •BMI between 18.5 - 30 kg/m2
排除标准
- •A potential subject who meets any of the following criteria will be excluded from participation in this study:
- •Involved in progressive exercise training
- •A history of neuromuscular problems
- •Use of anticoagulants
- •Recent (<12 months) participation in amino acid tracer (L-[ring-13C6] phenylalanineand L-[3,5-2H2]-tyrosine) studies
- •Use of medication known to affect (muscle) protein metabolism (e.g. corticosteroids, non-steroidal anti-inflammatory drugs, acne medication)
- •Phenylketonuria
- •Diagnosed with or history of liver damage
- •Diagnosed with or history of severe kidney damage and/or malfunction
- •Diagnosed with inability to break down amino acids
研究组 & 干预措施
Continuous amino acid infusion
Tracer infusions combined with infusion of a mixture of amino acids
干预措施: Continuous intravenous stable isotope amino acid tracer infusion (Other)
Continuous amino acid infusion
Tracer infusions combined with infusion of a mixture of amino acids
干预措施: Amino acid infusion (Other)
结局指标
主要结局
Muscle protein synthesis during continuous elevated plasma amino acid avaialbility in healthy young males
时间窗: 8 hours
The primary endpoint is muscle protein synthesis rates (in %/h) over the full assessment period (8 h) as determined with contemporary stable isotope tracer methodology combined with repeated blood and muscle sampling. Muscle protein synthesis are calculated using plasma as precursor pool and the tracer enrichment in the muscle (measured with UPLC and GC-IRMS).
次要结局
- Whole-body protein metabolism(8 hours)
- Whole-body protein kinetics(8 hours)
- Basal muscle protein synthesis rates(3 hours)