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Clinical Trials/NCT04150042
NCT04150042
Recruiting
Phase 1

SHARON: Study of Metastatic Cancers in Patients Using Autologous Stems Cells and Potentiated Redox Cycling to Overcome Drug Resistance to Nitrogen Mustard Derivatives

General Oncology, Inc.2 sites in 1 country24 target enrollmentJanuary 13, 2021

Overview

Phase
Phase 1
Intervention
Vitamin B12B
Conditions
Pancreatic Adenocarcinoma Metastatic
Sponsor
General Oncology, Inc.
Enrollment
24
Locations
2
Primary Endpoint
Overall incidence rate of adverse events
Status
Recruiting
Last Updated
3 months ago

Overview

Brief Summary

The clinical trial is a phase 1, single-arm trial that will evaluate the safety of the investigational treatment on metastatic pancreatic cancer and metastatic breast cancer. The investigational treatment will involve 2 cycles of a combination of intravenous melphalan, BCNU, vitamin B12b, and vitamin C with autologous hematopoietic stem cell infusion. A dose-escalation schedule is being employed for the vitamin C.

Detailed Description

In the current clinical trial, subjects will receive a combination of melphalan, BCNU, vitamin B12b, and vitamin C in conjunction with autologous stem cell infusion. The drug combination is designed to address multiple mechanisms of melphalan resistance. Investigational Treatment Description: * Hematopoietic Stem Cell Collection 1. Granulocyte colony-stimulating factor, and if needed Plerixafor, will be used to mobilize bone marrow stem cells, which will be collected by apheresis. 2. At least 2 bags of CD34+ cells, each containing at least 2 × 10\^6 cells/kg, will be prepared and stored. 3. Mobilization of hematopoietic stem cells will only occur prior to the first cycle of investigational therapy. 4. If there is not a sufficient mobilization of stem cells for at least 2 cycles of chemotherapy, then no investigational drugs will be given. * Investigational Drug Therapy and Stem Cell Infusion 1. All subjects will receive two cycles of investigational drug therapy with stem cell infusion unless precluded by adverse reactions. 2. Subjects will receive on day -2: 1. BCNU 2. Melphalan 3. Vitamin B12b 4. Vitamin C 3. On day 0, at least 2 × 10\^6 CD34+ cells/kg will be infused as per the institution's standard procedures. 4. Subjects will receive supportive care as per the institution's standard procedures before, during, and after the investigational drug therapy and stem cell infusion. * Additional Cycles a. Subjects will receive a second cycle of the investigational treatment described immediately above in "Investigational Drug Therapy and Stem Cell Infusion," with an interval of approximately 6 weeks between cycles.

Registry
clinicaltrials.gov
Start Date
January 13, 2021
End Date
December 1, 2028
Last Updated
3 months ago
Study Type
Interventional
Study Design
Single Group
Sex
All

Investigators

Responsible Party
Sponsor

Eligibility Criteria

Inclusion Criteria

  • Age ≥ 18 years.
  • Pancreatic or breast cancer, as described below.
  • Stage IV (based on AJCC staging guidelines) at the time of enrollment.
  • a. Note that potential subjects with stage IV cancer that have had a complete response from prior chemotherapy are still potentially eligible.
  • Expected survival time ≥ 6 months, as determined by the investigator.
  • Life expectancy not severely limited by diseases other than malignancy, as determined by the investigator.
  • Karnofsky score ≥ 60%.
  • No chemotherapy within 2 weeks of enrollment.
  • Prior surgical resection or ablation of the primary tumor is allowed but not required.
  • If post-surgical, the subject must be at least 28 days post-op with the surgical wounds healed and significant complications resolved.

Exclusion Criteria

  • Rapid disease progression or clinical features concerning for onset of rapid symptomatic deterioration, as determined by the investigator.
  • Biliary tract obstruction.
  • Current cholangitis. A biliary stent in situ does not otherwise exclude protocol participation.
  • A history of only one episode of cholangitis and fewer than 30 days have passed since discontinuation of antibiotic treatment.
  • A history of multiple episodes of cholangitis and after discussion between the site study team and sponsor medical monitor and careful evaluation for suitability the patient is deemed to be unsuitable for the trial due to risk of recurring cholangitis.
  • Portal hypertension.
  • Sinistral portal hypertension.
  • Obliteration or significant obstruction of the major veins or arteries (e.g., portal vein, superior mesenteric artery, superior mesenteric vein).
  • Clinically significant malignant ascites or malignant pleural effusion, as determined by the investigator.
  • Metastatic lesion to the heart or eye.

Arms & Interventions

Chemotherapy/stem cell treatment

Intervention: Vitamin B12B

Chemotherapy/stem cell treatment

Intervention: Vitamin C

Chemotherapy/stem cell treatment

Intervention: Melphalan

Chemotherapy/stem cell treatment

Intervention: BCNU

Chemotherapy/stem cell treatment

Intervention: Autologous Hematopoietic Stem Cells

Outcomes

Primary Outcomes

Overall incidence rate of adverse events

Time Frame: Until 12 months after the second stem cell treatment

Adverse event is defined any untoward medical occurrence associated with the use of a drug in humans, whether or not considered drug-related.

Overall incidence rate of serious adverse events

Time Frame: Until 12 months after the second stem cell treatment

An adverse event is considered serious if, in the view of either the investigator or Sponsor, it results in any of the following outcomes: * Death. * A life-threatening adverse event. * Inpatient hospitalization or prolongation of existing hospitalization. * A persistent or significant incapacity or substantial disruption of the ability to conduct normal life functions. * A congenital anomaly or birth defect. Important medical events that may not result in death, be life-threatening, or require hospitalization may be considered serious when, based upon appropriate medical judgment, they may jeopardize the patient or subject and may require medical or surgical intervention to prevent one of the outcomes listed in this definition.

Rate of Sinusoidal obstruction syndrome

Time Frame: 30 days after treatment

Sinusoidal obstruction syndrome diagnosis and grading will use the European Society for Blood and Marrow Transplantation's Revised Diagnosis and Severity Criteria for Sinusoidal Obstruction Syndrome/Veno-Occlusive Disease in Adult Patients as published in 2016. Gradings are from mild to very severe (multi-organ dysfunction/multi-organ failure).

Overall incidence rate of Grade 3-5 adverse events

Time Frame: Until 12 months after the second stem cell treatment

Grading will be measured using Common Terminology Criteria for Adverse Events version 5.0

Rate of Presumptive Oxalate Nephropathy

Time Frame: Within 48 hours of vitamin C treatment

Oxalate nephropathy will be presumed if there is acute kidney injury or increased creatinine, grade 3 or higher by the criteria of CTCAE Version 5.0 within 48 h of the administration of vitamin C, in the absence of a clear alternative explanation (an example of an alternative explanation is tumor lysis syndrome).

Rate of Delayed Engraftment of Neutrophils

Time Frame: Day 21 after each treatment

Neutrophil engraftment is defined as an absolute neutrophil count ≥ 500/microliter for 3 days, with the date of engraftment being the first of those 3 days. Delayed engraftment is engraftment that occurs after 21 days but within 30 days.

Rate of Mucositis ≥ Grade 3

Time Frame: Day 21 after each treatment

Mucositis will be assessed using the WHO Mucositis Scale. Grading is from 0 (no symptoms) to 4 (no possible alimentation).

Rate of Failed Engraftment of Neutrophils

Time Frame: Day 30 after each treatment

Neutrophil engraftment is defined as an absolute neutrophil count ≥ 500/microliter for 3 days, with the date of engraftment being the first of those 3 days. Failure to engraft within 30 days will be considered an engraftment failure.

Rate of Delayed Engraftment of Platelets

Time Frame: Day 30 after each treatment

Platelet engraftment is defined as a platelet count ≥ 20,000/microliter for 3 days, with the date of engraftment being the first of those 3 days. Delayed engraftment is engraftment that occurs after 30 days.

Rate of Idiopathic or Non-Infective Pulmonary Toxicity ≥ Grade 3

Time Frame: 3 months after the last treatment

The American Thoracic Society Committee on Idiopathic Pneumonia Syndrome definition will be employed.

Rate of Idiopathic or Non-Infective Pulmonary Toxicity ≥ Grade 3

Time Frame: 6 months after the last treatment

The American Thoracic Society Committee on Idiopathic Pneumonia Syndrome definition will be employed.

Rate of Cytokine Release Syndrome ≥ Grade 3

Time Frame: Within 48 hours of each vitamin C treatment

Cytokine release syndrome will be assessed by the criteria of CTCAE Version 5.0. Elevation of plasma cytokine levels consistent with the diagnosis of cytokine release syndrome must be present.

Rate of Mucositis ≥ Grade 3

Time Frame: Day 7 after each treatment

Mucositis will be assessed using the WHO Mucositis Scale. Grading is from 0 (no symptoms) to 4 (no possible alimentation).

Rate of Mucositis ≥ Grade 3

Time Frame: Day 14 after each treatment

Mucositis will be assessed using the WHO Mucositis Scale. Grading is from 0 (no symptoms) to 4 (no possible alimentation).

Secondary Outcomes

  • Overall Survival(Until 12 months after the second stem cell treatment)
  • Progression-Free Survival(Until 12 months after the second stem cell treatment)
  • Objective response according to RECIST version 1.1(12 months after the second stem cell treatment)
  • Objective response rate in metastatic lesions(12 months after the second stem cell treatment)
  • Objective response according to RECIST version 1.1(1 month after the first stem cell treatment)
  • Objective response according to RECIST version 1.1(1 month after the second stem cell treatment)
  • Objective response according to RECIST version 1.1(3 months after the second stem cell treatment)
  • Objective response according to RECIST version 1.1(6 months after the second stem cell treatment)
  • Objective response according to RECIST version 1.1(9 months after the second stem cell treatment)
  • Objective response rate in metastatic lesions(1 month after the first stem cell treatment)
  • Objective response rate in metastatic lesions(1 month after the second stem cell treatment)
  • Objective response rate in metastatic lesions(3 months after the second stem cell treatment)
  • Objective response rate in metastatic lesions(6 months after the second stem cell treatment)
  • Objective response rate in metastatic lesions(9 months after the second stem cell treatment)

Study Sites (2)

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SHARON Trial Shows Promising Results for BRCA-Mutated Pancreatic Cancer Using Stem Cell Transplant- The phase 1 SHARON trial demonstrated that melphalan, BCNU, hydroxocobalamin, and ascorbic acid followed by autologous stem cell transplant was safe and feasible for stage IV pancreatic cancer patients with BRCA1/2 or PALB2 mutations. - Patients achieved a median progression-free survival of 14.2 months, nearly doubling the 7.5 months seen in the POLO trial with olaparib, despite having more aggressive disease and prior treatment lines. - The treatment showed a 100% disease control rate in non-progressing patients, with 40% achieving durable response free of disease after transplant. - Researchers plan to expand enrollment by 15-20 more patients in phase 1 before advancing to phase 2, focusing on patients not progressing on chemotherapy.General Oncology to Present Phase 1 SHARON Trial Results for BRCA/PALB2-Mutated Cancers at ESMO 2025- General Oncology will present preliminary Phase 1 results from the SHARON trial evaluating GO-4 therapy in patients with BRCA/PALB2-mutated pancreatic and breast cancers at ESMO 2025. - The GO-4 regimen combines melphalan, BCNU, hydroxocobalamin, ascorbic acid, and autologous stem cell infusion as an Advanced Redox Modulation approach for metastatic cancers. - The Phase 1 study is designed to evaluate safety, feasibility, and preliminary efficacy in patients with advanced pancreatic ductal adenocarcinoma or breast cancer. - The trial will expand enrollment to include patients both with and without inherited BRCA/PALB2 mutations for the remainder of the study.