Nutritional Study:The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 14
- 试验地点
- 1
- 主要终点
- Change in plasma MDA levels following meat meal
研究概览
简要总结
The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adults, normal BMI
排除标准
- •Metabolic disorders
- •Taking nutritional supplement
研究组 & 干预措施
frozen meat
250 g of frozen meat meal for 4 days
干预措施: frozen meat cutlets meal (Other)
frozen meat
250 g of frozen meat meal for 4 days
干预措施: meat meals (Other)
frozen meat and wine
250 g of frozen meat and red wine for 4 days
干预措施: frozen meat cutlets meal (Other)
frozen meat and wine
250 g of frozen meat and red wine for 4 days
干预措施: meat meals (Other)
fresh meat
250 g of fresh meat meal
干预措施: frozen meat cutlets meal (Other)
fresh meat
250 g of fresh meat meal
干预措施: meat meals (Other)
fresh meat and wine
250 g of fresh meat meal
干预措施: frozen meat cutlets meal (Other)
fresh meat and wine
250 g of fresh meat meal
干预措施: meat meals (Other)
结局指标
主要结局
Change in plasma MDA levels following meat meal
时间窗: Fasting before meal and 3, and 6 h postprandial, and fasting levels
After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples
Changes in MDA concentration on LDL fraction following meat meals
时间窗: Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast
Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC.
次要结局
未报告次要终点